1887

Abstract

SUMMARY: Among 20 locally isolated fungi, isolate no. 58 proved to be suitable for the production of active extracellular milk-clotting enzyme. The addition of acetate buffer to the reaction mixture enhanced the clotting of milk by the enzyme whereas citrate + phosphate buffer hindered the process. The general properties of the crude enzyme were studied. Precipitation with ammonium sulphate, ethanol, acetone and tannin showed that ammonium sulphate was unsuitable for precipitation while the other precipitants produced sufficiently active fractions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-59-1-125
1969-11-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/59/1/mic-59-1-125.html?itemId=/content/journal/micro/10.1099/00221287-59-1-125&mimeType=html&fmt=ahah

References

  1. Arima K., Iwasaki S. 1964; Milk coagulating enzyme ’microbial rennet’ and method of preparation thereof.. U.S.Pat 3:151039
    [Google Scholar]
  2. Bergkvist R. 1963; The proteolytic enzymes of Aspergillus oryzae. I. Methods for the estimation and isolation of the proteolytic enzymes.. Acta chem. scand 17:1521
    [Google Scholar]
  3. Berridge N. J. 1952; Some observations on the determination of the activity of rennet.. Analyst, Lond 77:57
    [Google Scholar]
  4. Iwasaki S., Tamura G., Arima K. 1967; Milk clotting enzyme from micro-organisms. II. The enzyme production and the properties of crude enzyme.. Agric. biol. Chem 31:546
    [Google Scholar]
  5. Knight S. G. 1966; Production of a rennin-like enzyme by moulds.. Can. J. Microbiol 12:420
    [Google Scholar]
  6. Prill E. A., Wenck P. R., Peterson W. H. 1935; Factors influencing the amount and nature of the fat produced by Aspergillus fischeri. . Biochem. J 29:21
    [Google Scholar]
  7. Tsugo T., Yoshino U., Taniguchi K., Ozawa A., Miki Y., Iwasaki S., Arima K. 1964; Studies on cheese making by using milk clotting enzyme of Mucor pusillus Lindt. I. Rennetic properties of enzyme.. Jap. J. zootech. Sci 35:221
    [Google Scholar]
  8. Veselov I., Tipograph D., Petina T. A. 1965; Aspergillus candidus as a producer of rennin.. Prikl. Biokhim. Mikrobiol 1:52
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-1-125
Loading
/content/journal/micro/10.1099/00221287-59-1-125
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error