1887

Abstract

SUMMARY: Among 20 locally isolated fungi, isolate no. 58 proved to be suitable for the production of active extracellular milk-clotting enzyme. The addition of acetate buffer to the reaction mixture enhanced the clotting of milk by the enzyme whereas citrate + phosphate buffer hindered the process. The general properties of the crude enzyme were studied. Precipitation with ammonium sulphate, ethanol, acetone and tannin showed that ammonium sulphate was unsuitable for precipitation while the other precipitants produced sufficiently active fractions.

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/content/journal/micro/10.1099/00221287-59-1-125
1969-11-01
2020-01-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-59-1-125
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