1887

Abstract

SUMMARY

L-phase variants of and resembled mycoplasmas in their ability to incorporate considerable amounts of exogenous cholesterol into their cell membranes. Much smaller quantities were incorporated by L-phase variants of and , by the parent bacteria of all L-phase variants except , and by a series of wall-covered bacteria, including their protoplasts and spheroplasts. Cholesterol-binding ability corresponded roughly to sensitivity to growth inhibition and lysis by digitonin. Like myco-plasma, the L-phase had a non-enzymic cholesterol uptake mechanism, but its growth in a cholesterol-free medium was not improved by adding cholesterol. It is concluded that a bacterium, on transforming to its stable L-phase, does not necessarily develop the ability to bind cholesterol; if it does, the cholesterol bound is not essential for growth of the L-phase, unlike cholesterol-requiring mycoplasmas.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-58-3-327
1969-11-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/58/3/mic-58-3-327.html?itemId=/content/journal/micro/10.1099/00221287-58-3-327&mimeType=html&fmt=ahah

References

  1. Argaman M., Razin S. 1965; Cholesterol and cholesterol esters in Mycoplasma. J. gen. Micro-biol 38:153
    [Google Scholar]
  2. Ashworth L. A. E., Green C. 1966; Plasma membranes: phospholipid and sterol content. Science, N.Y 151:210
    [Google Scholar]
  3. Birdsell D. C., Cota-Robles E. H. 1967; Production and ultrastructure of lysozyme and ethy-lenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J. Bact 93:427
    [Google Scholar]
  4. Van Boven C.P.A., Kastelein M. J. W., Hijmans W. 1967; A chemically defined medium for the L-phase of group A streptococci. Ann. N. Y. Acad. Sci 143:749
    [Google Scholar]
  5. Bruckdorfer K. R., Graham J. M., Green C. 1968; The incorporation of steroid molecules into lecithin sols, β-lipoproteins and cellular membranes. European J. Biochem 4:512
    [Google Scholar]
  6. Burmeister H. R., Hesseltine C. W. 1968; Induction and propagation of a Bacillus subtilis L-form in natural and synthetic media. J. Bact 95:1857
    [Google Scholar]
  7. Chu H. P., Horne R. W. 1967; Electron microscopy of Mycoplasma gallisepticum and Mycoplasma mycoides using the negative staining technique and their comparison with myxovirus. Ann. N.Y. Acad. Sci 143:190
    [Google Scholar]
  8. De Gier J., Mandersloot J. G., Van Deenen L. L. M. 1969; The role of cholesterol in lipid membranes. Biochim. biophys. Acta 173:143
    [Google Scholar]
  9. Dienes L. 1939; L-organisms of Klieneberger and Streptobacillus moniliformis. J. infect. Dis 65:24
    [Google Scholar]
  10. Edman D. C., Pollock M. B., Hall E. R. 1968; Listeria monocytogenes L forms. I. Induction, maintenance, and biological characteristics. J. Bact 96:352
    [Google Scholar]
  11. Edward D. G. ff. 1953; A difference in growth requirements between bacteria in the L-phase and organisms of the pleuropneumonia group. J. gen. Microbiol 8:256
    [Google Scholar]
  12. Fiertel A., Klein H. P. 1959; On sterols in bacteria. J. Bact 78:738
    [Google Scholar]
  13. Gilby A. R., Few A. V. 1960; Lysis of protoplasts of Micrococcus lysodeikticus by ionic detergents. J. gen. Microbiol 23:19
    [Google Scholar]
  14. Graham J. M., Green C. 1967; The binding of sterols in cellular membranes. Biochem. J 103:16c
    [Google Scholar]
  15. Klein S. J., Stone F. M. The lysis of pneumococcus by saponin. J. Bact 22:387
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  17. MacLeod C. M. 1965 Bacterial and Mycotic Infections of Man, 4. Dubos R. J., and Hirsch J. G. Philadelphia: J. B. Lippincott;
    [Google Scholar]
  18. Miller D. M. 1963; ‘Flickering’ in protoplasts of Bacillus megaterium. Science, N. Y 139:1060
    [Google Scholar]
  19. Nelson G. J. 1967; Composition of neutral lipids from erythrocytes of common mammals. J. Lipid Res 8:374
    [Google Scholar]
  20. Partridge S. M., Klieneberger E. 1941; Isolation of cholesterol from the oily droplets found in association with the L 1 organism separated from Streptobacillus moniliformis. J. Path. Bact 52:219
    [Google Scholar]
  21. Pfleger R. C., Anderson N. G., Snyder F. 1968; Lipid class and fatty acid composition of rat liver plasma membranes isolated by zonal centrifugation. Biochemistry 7:2826
    [Google Scholar]
  22. Razin S. 1963; Osmotic lysis of Mycoplasma. J. gen. Microbiol 33:471
    [Google Scholar]
  23. Razin S. 1967; The cell membrane of Mycoplasma. Ann. N. Y. Acad. Sci 143:115
    [Google Scholar]
  24. Razin S. 1969; The mycoplasma membrane. In The Mycoplasmatales and the L-phase of Bacteria Hayflick L. 317 New York: Appleton Century Crofts Inc;
    [Google Scholar]
  25. Razin S., Argaman M. 1963; Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents. J. gen. Microbiol 30:155
    [Google Scholar]
  26. Razin S., Boschwttz H. 1968; The membrane of the Streptobacillus moniliformis L-phase. J. gen. Microbiol 54:21
    [Google Scholar]
  27. Razin S., Cohen A. 1963; Nutritional requirements and metabolism of Mycoplasma laidlawii. J. gen. Microbiol 30:141
    [Google Scholar]
  28. Razin S., Morowitz H. J., Terry T. M. 1965; Membrane subunits of Mycoplasma laidlawii and their assembly to membranelike structures. Proc. natn. Acad. SciU.S.A 54219
    [Google Scholar]
  29. Rebel G., Bader-Hirsch A. M., Mandel P. 1963; Recherches sur les lipides des formes L. dérivées du Proteus P 18.1. Presence et absorption du cholesterol. Bull. Soc. Chim. biol 45:1327
    [Google Scholar]
  30. Rothblat G. H., Hartzell R. W. Jun., Miahle H., Kritchevsky D. 1966; The uptake of cholesterol by L5178 Y tissue-culture cells: studies with free cholesterol. Biochim. biophys. Acta 116:133
    [Google Scholar]
  31. Rothfield L., Finkepstein A. 1968; Membrane biochemistry. A. Rev. Biochem 37:463
    [Google Scholar]
  32. Santaolalla M. 1966; Estudio comparativo del componente graso de Agrobacterium tumefaciens Y de sus formas L fijas. II. Variaciones según el medio de cultivo y estudio de los fosfolipidos. Microbiologia esp 19:1
    [Google Scholar]
  33. Smith P. F., Rothblat G. H. 1960; Incorporation of cholesterol by pleuropneumonia-like organisms. J. Bact 80:842
    [Google Scholar]
  34. Smith P. F., Rothblat G. H. 1962; Comparison of lipid composition of pleuropneumonia-like and L-type organisms. J. Bact 83:500
    [Google Scholar]
  35. Willmer E. N. 1961; Steroids and cell surfaces. Biol. Rev 36:368
    [Google Scholar]
  36. Mcgee Z. A., Rogul M., Wittler R. G. 1967; Molecular genetic studies of relationships among mycoplasma, L-forms and bacteria. Ann. N. Y. Acad. Set 143:21
    [Google Scholar]
  37. Razin S. 1968; Mycoplasma taxonomy studied by electrophoresis of cell proteins. J. Bact 96:687
    [Google Scholar]
  38. Razin S., Rottem S. 1967; Identification of Mycoplasma and other micro-organisms by poly- acrylamide-gel electrophoresis of cell proteins. J. Bact 94:1807
    [Google Scholar]
  39. Sacks T., Haas H., Razin S. 1969; Polyacrylamide-gel electrophoresis of cell proteins of Enterobacteriaceae. A possible taxonomic aid. Israel J. Med. Sci 5:49
    [Google Scholar]
  40. Theodore T. S., King J. R., Cole R. M. 1969; Identification of L forms by polyacrylamide-gel electrophoresis. J. Bact 97:495
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-58-3-327
Loading
/content/journal/micro/10.1099/00221287-58-3-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error