SUMMARY: Washed suspensions of grown and incubated anaerobically incorporated C from [C]glucose into the cell pool as [C]glucose, maltose and hexose phosphate and into protozoal polysaccharide and intracellular bacteria as a glucose polymer. The uptake of sugars into the pool was by an active process (predominant at low sugar concentrations) probably into the protozoal endoplasm, and by a passive process (predominant at high concentrations) into another part of the cell. Uptake of glucose by the active process was not sufficiently rapid to increase the level of glucose in the pool by more than 17% per hour; this was probably insufficient to increase appreciably the rate of glucose utilization. These protozoa hydrolysed starch to maltose and glucose and the level of these sugars in the pool of protozoa metabolizing starch grains was controlled in part by the inhibitory effect of these sugars on the enzymes that formed them. Studies on the enzymes involved in the metabolism of starch showed that the glucose in starch was hydrolysed to free glucose before phosphorylation. In experiments on the effect of carbohydrates on the survival of protozoa, starch was the most effective in prolonging protozoal life, followed by maltose and glucose in that order. The greater effectiveness of maltose was correlated with the finding that the rate of uptake of maltose carbon was always greater than that for glucose when the two sugars were present in equimolar concentrations. However, the rate for both sugars was markedly decreased in the presence of penicillin and neomycin. Evidence is presented that the protozoal ectoplasm may be freely permeable to sugars in the medium and that there is a barrier between the ectoplasm and endoplasm. C from [C]starch grains was incorporated by the protozoa but there was no synthesis of protozoal protein from carbohydrate.


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