SUMMARY: Cells of grown with or without competence promoting factors, inosine and lactate, were compared in their ability to bind deoxyribonucleic acid (DNA) and to undergo a genetic transformation. It was found that cells grown with the above factors bound 70 % of the C-labelled DNA and transformed to a relatively high level (1·4 to 2·5 %). Cells grown without the factors bound only 8 % of the C-labelled DNA and transformed to a low level (0·01 % or less).

It is suggested from these studies that during growth cells of use inosine and lactate either to form or modify that portion of the cell surface that can later become involved in binding DNA.


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