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The guanine + cytosine (GC) base compositions of deoxyribonucleic acids (DNA) of avian mycoplasmas, as well as strains from other sources, were determined from buoyant density in CsCl. The values from our work ranged from 24·0 to 35·7 moles%. There were small clusters at about 35% and 32% but the rest of the values ranged continuously from 24·0% to 30·5%. From reports in the literature, the values for the Mycoplasma group ranged from 22·8 to 41·0.
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