1887

Abstract

SUMMARY: Phage N6 lysates of and were very viscous and were found to mediate genetic transfer of an adenine marker (ade) to adenine-dependent strains of The transfer activity of these lysates was found to reside in a high molecular weight DNA fraction rather than in the phage particles themselves. DNA that was isolated and purified from phage lysates and lysozyme-treated cells was shown to possess transforming activity. Recipient competence was maximal during late logarithmic growth (14–18 hr). The cell density of the transfer suspension was optimum at about 10 colony forming units/ml. Transformants were recovered after 1 hr exposure to DNA and continued to increase in number up to 6 hr. Transformants appeared in higher frequencies on Difco agars that were less purified. Under optimal conditions, transformation frequencies up to several percent per colony forming unit were obtained. All strains of tested ( 4698, 15801, 1335, , , , ) could serve as donors of the ade marker. All strains, except 4698, were competent recipients of the adenine marker.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-55-2-307
1969-02-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/55/2/mic-55-2-307.html?itemId=/content/journal/micro/10.1099/00221287-55-2-307&mimeType=html&fmt=ahah

References

  1. Brown A. H. 1946; Determination of pentose in the presence of large quantities of glucose. Archs. Biochem 11:269
    [Google Scholar]
  2. Burgi E. 1955 A study of abortive infection in a mutant strain of Micrococcus lysodeikticus infected with a bacteriophage. Ph.D thesis Cornell University, New York:
    [Google Scholar]
  3. Campbell J. N., Evans J. B., Perry J. J., Niven C. F. Jun. 1961; An extracellular material elaborated by Micrococcus sodonensis . J. Bact 82:828
    [Google Scholar]
  4. Demerec M. E., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61
    [Google Scholar]
  5. Dische Z. 1955; Color reactions of nucleic acid components. In The Nucleic Acids. Chargaff E., Davidson J.N. Ed. by 1 p. 285 New York and London: Academic Press;
    [Google Scholar]
  6. Feiner R. R. 1967; Ultraviolet radiation-sensitive mutants of Micrococcus lysodeikticus . J. Bact 94:1270
    [Google Scholar]
  7. Feiner R. R., Meyer K., Steinberg A. 1946; Bacterial lysis by lysozyme. J. Bact 52:375
    [Google Scholar]
  8. Fleming A. 1922; On a remarkable bacteriolytic element found in tissues and secretions. Proc. R. Soc B 93:306
    [Google Scholar]
  9. Fleming A., Allison V. D. 1927; On the development of strains of bacteria resistant to lysozyme action and the relation of lysozyme action to intracellular digestion. Br. J. exp. Path 8:214
    [Google Scholar]
  10. Grula E. A. 1962; A comparative study of six cultures of Micrococcus lysodeikticus . Can. J. Microbiol 8:855
    [Google Scholar]
  11. Kloos W. E. 1968; Evidence of genetic exchange in Micrococcus lysodeikticus . Bact. Proc. p. 55
    [Google Scholar]
  12. Kocur M., Martinec R. 1962; A taxonomic study of the genus Micrococcus . Folia Fac. Sci. Natur. Univ Purkynianae Brunensis 3:1
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol 3:208
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. molec. Biol 5:109
    [Google Scholar]
  16. Mejbaum W. 1939; Über die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylsaure. Z. physiol. Chem 258:117
    [Google Scholar]
  17. Naylor H. B., Burgi E. 1956; Observations on abortive infection of Micrococcus lysodeikticus with bacteriophage. Virology 2:577
    [Google Scholar]
  18. Okubo S., Nakayama H., Sekiguchi M., Takagi Y. 1967; A mutant of Micrococcus lyso-deikticus defective in a deoxyribonuclease activity specific for ultraviolet-irradiated DNA. Biochem. biophys. Res. Commun 27:224
    [Google Scholar]
  19. Rosypalova A., Bohacek J., Rosypal S. 1966; Deoxyribonucleic acid base composition of some micrococci and sarcinae. Antonie van Leeuwenhoek 32:192
    [Google Scholar]
  20. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treat-ment. Biochem. biophys, Acta 72:619
    [Google Scholar]
  21. Stechschulte A. L. 1961; Isolation of biochemical and chromogenic mutants of Micrococcus lysodeikticus using lysozyme as the selective agent. Biol. Ser. Cath. Univ. Am.68
    [Google Scholar]
  22. Swanstrom M., Adams M. H. 1951; Agar layer method of production of high titre phage stocks. Proc. Soc. expt. Biol. Med 78:372
    [Google Scholar]
  23. Wolin H. L., Naylor H. B. 1957; Basic nutritional requirements of Micrococcus lysodeikticus . J. Bact 74:163
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-55-2-307
Loading
/content/journal/micro/10.1099/00221287-55-2-307
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error