SUMMARY: Three heterotrophic marine bacteria, isolated from the Tyrrhenian sea on the basis of their relative growth requirements for acetate and ammonium, were studied mainly in regard to their biochemical and nutritional characters. Data about the amino acid pools, protein amino acids, CO:O ratios, enzyme activities and sensitivities to metal ions and antibiotics, are reported. The utility of an index obtained from the study of the oxygen uptake with various organic-C concentrations and the use of defined media for a more satisfactory discrimination of the various strains, is shown.


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