SUMMARY: An organism was isolated in pure culture from germinating seeds of Brem. By standard bacteriological techniques the organism was considered to belong to the group. It was shown to fix nitrogen. By using an antiserum prepared against one strain, four strains were found to be serologically identical and showed some cross-reaction with . An immunofluorescence technique was used in a search for these organisms in the seed embryo, leaf nodules and other tissues of a number of leaf-nodulated Rubiaceae and Myrsinaceae including and a non- nodulated species of Myrsinaceae, . From the results obtained it is concluded that this serotype of is present in the leaf-nodulated members of these families of plants.


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