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SUMMARY: The addition of sodium-L-glutamate (1%, w/v) to the Mueller medium in which Clostridium tetani was grown initially stimulated growth and toxin production, then caused early lysis, and resulted finally in a marked suppression in the titre of tetanus toxin in the culture measured after 7 days. The suppression of production of tetanus toxin was not associated with any general suppression of protein production or with a decrease in the production of any major antigen.
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