1887

Abstract

SUMMARY: F donor bacteria were treated with u.v. radiation, mitomycin C, nitrous acid, hydroxylamine or caffeine, and then incubated for various periods in broth at 37° before conjugation. U.v. radiation, mitomycin C and nitrous acid increased the fertility of F populations, but hydroxylamine and caffeine did not. The maximal effect of u.v. irradiation was obtained after using a dose 50-100 ergs/mm. and after 45 min. incubation in broth of irradiated bacteria before conjugation. The maximal effect of mitomycin C occurred after treatment with 1‐3 µg./ml. and after 110 min. incubation in broth. Depending on the strain used, the fertility of the population increased 8-20 times after u.v. irradiation and 5-21 times after mitomycin C. The effect of u.v. irradiation could be photoreactivated up to 50%. Following treatment of strain 1655 with nitrous acid (0·01 ), a three-to four-fold increase in the yield of conjugation was obtained, although irregularly. Caffeine (0·01%) enhanced the effect of u.v. irradiation about two-fold. Chloramphenicol blocked the expression of the effect of u.v. irradiation and mitomycin C. The effect of chloramphenicol was not permanent, because after its removal followed by further incubation the fertility of the F population increased to the same degree as in the samples in which the bacteria, previously exposed to u.v. radiation or mitomycin C, were incubated in broth without chloramphenicol.

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1968-11-01
2024-04-26
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References

  1. ADELBERG E. A. , PITTARD J. . 1965; Chromosome transfer in bacterial conjugation.. Bact. Rev26–161
    [Google Scholar]
  2. BOYCE R. P., HOWARD-FLANDERS P. 1964a; Release of ultraviolet light-induced thymine dimers from DNA in E. coli K 12.. Proc. natn. Acad. Sci.,; U.S.A.:51–293
    [Google Scholar]
  3. BOYCE R. P., HOWARD-FLANDERS P. 1964b; Genetic control of DNA breakdown and repair in E. coli K 12 treated with mitomycin C or ultraviolet light.. Z. Vererb. Lehre 95345
    [Google Scholar]
  4. DOBRZANSKI W. T. 1964; Thesis: O dziafoniu letalnym i mutagennym promieniowania UV na szczep gronkowca ziocistego 209P.. Osrodek Wydawnictw Akademii Medycznej w Warszawiep.102
    [Google Scholar]
  5. DOBRZANSKI W. T. 1965; Dziatanie letalne i mutagenne promieniowania ultrafioletowego na bakterie.. Post. Mikrobiol4–3
    [Google Scholar]
  6. DRISKEL-ZAMENHOF P.J., ADELBERG E. A. 1963; Studies on the chemical nature and size of the sex factors of E. coli K 12.. J . molec. Biol6–483
    [Google Scholar]
  7. GEIDUSCHEK E. P. 1961; ‘Reversible’ DNA.. Proc. natn. Acad. Sci.; U.S.:47–950
    [Google Scholar]
  8. HAYES W. 1952a; Recombination in E. coli k 12: unidirectional transfer of genetic material.. Nature, Lond169–118
    [Google Scholar]
  9. HAYES W. 1952b; Genetic recombination in Bact. coli K 12: analysis of the stimulation effect of ultraviolet light.. Nature, Lond169–1017
    [Google Scholar]
  10. HAYES W. 1953; Observations on transmissible agent determining sexual differentiation.. J. gen. Microbiol8–72
    [Google Scholar]
  11. HAYES W. 1964; The Genetics of Bacteria and their Viruses.. Oxford:: Blackwell Scientific Publications.;
    [Google Scholar]
  12. HOWARD-FLANDERS P., BOYCE R. P., THERRIOT L. 1966; Three loci in Escherichia coli K 12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.. Genetics53–1119
    [Google Scholar]
  13. Jacob F., Wollman E. L. 1956; Recombinaison genetique et mutants de fertilite chez E. coli.. C. r. hebd. Seanc. Acad. Sci.,; Paris:242–303
    [Google Scholar]
  14. LEDERBERG J. 1950; Isolation and characterization of biochemical mutants of bacteria.. Meth. med. Res.3–5
    [Google Scholar]
  15. Lieb M. 1961; Enhancement of UV-induced mutation in bacteria by caffeine.. Z. Vererb. Lehre 92416
    [Google Scholar]
  16. MARMUR J., ANDERSON W. F., MATTEWS L., BERNS K., GAJEWSKA E., LANE D., DOTY P. 1961; The effect of ultraviolet light on the biological and physical chemical properties of deoxyribonucleic acids.. J. cell. comp. Physiol. 58, Suppl1–33
    [Google Scholar]
  17. METZGER K. 1964; On the dark-reactivation mechanism in ultraviolet irradiated bacteria.. Biochem. biophys. Res. Comm15–101
    [Google Scholar]
  18. RAJCHERT-TRZPIL M. 1965; The influence of chloramphenicol on the conjugation yield in E. coli K 12. Bull.. Acad polon.sci. Cl. VI13–211
    [Google Scholar]
  19. REICH E., SHATKIN A. J., TATUM E. L. 1961; Bacteriocidal action of mitomycin C.. Biochim. biophys. Acta53–132
    [Google Scholar]
  20. SCAIFE J., GROSS J. D. 1963; The mechanism of chromosome mobilization by an F-prime factor in E. coli K 12.. Genetic Res. Camb4–328
    [Google Scholar]
  21. SETLOW J. K., BOLING M. E., BOLLUM E. J. 1965; The chemical nature of photoreactivable lesions in DNA.. Proc. natn. Acad. Sci.; U.S.:53–1430
    [Google Scholar]
  22. STERN C. 1963; Methodology in Basic Genetics.. Ed. by Burdette W. L. San Francisco:: Holden-Day, Inc;
    [Google Scholar]
  23. SZYBALSKI W., IYER V. N. 1964; Crosslinking of DNA by enzymatically or chemically activated mitomycins and porfiromycins, bifunctionally ‘alkylating’ antibiotics. Fed. Proc. Fedn. Am. Socs exp. Biol23–946
    [Google Scholar]
  24. TESSMAN I. 1962; The induction of large deletions by nitrous acid. J. molec. Biol5–442
    [Google Scholar]
  25. TESSMAN I., PODDAR R. K., KUMAR S. 1964; Identification of the altered bases in mutated single- stranded DNA. I. In vitro mutagenesis by hydroxylamine, ethylmethanesulfonate and nitrous acid. J. molec. Biol9–352
    [Google Scholar]
  26. WOLLMAN E. L., JACOB F., HAYES W. 1956; Conjugation and genetic recombination in E. coli K 12. Cold. Spring Harb. Symp. quant. Biol21–141
    [Google Scholar]
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