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SUMMARY: Estimation of chromosome numbers in approximately 1000 nuclei of amoebae and plasmodia of the true slime mould, Didymium nigripes, has shown that in the c6 and s3 strains the ploidy level of amoebae and plasmodia is the same. Nuclei with differing chromosome numbers were found in the populations studied. The data collected here are consistent with the hypothesis that as a culture ages during serial subculture of one stage of the life-cycle, nuclei with higher than normal chromosome numbers accumulate.
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