1887

Abstract

SUMMARY: Yeast protoplasts which were in osmotic equilibrium with a 0.55 M-mannitol solution underwent lysis when glucose was added to the suspension. Lysis was dependent on glucose metabolism and was accelerated when chelating agents were added to the medium. Lysis in the presence of glucose and chelating agents was inhibitied by alkali metal ions, magnesium, spermidine and by increasing the tonicity of the test medium. The inhibition of lysis by K and Na when chelating agents were present conformed to a Michaelis-Menten type relationship with apparent dissociation constants of 2–5 × 10–4 M and 3–6 × 10 M for K and Na, respectively.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-51-3-433
1968-05-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/51/3/mic-51-3-433.html?itemId=/content/journal/micro/10.1099/00221287-51-3-433&mimeType=html&fmt=ahah

References

  1. Abrams A. 1959; Reversible metabolic swelling of bacterial protoplasts. J. biol. Chem 234:383
    [Google Scholar]
  2. Abrams A. 1960; Metabolically dependent penetration of oligosaccharides into bacterial cells and protoplasts. J. biol Chem 235:1281
    [Google Scholar]
  3. Armstrong W. McD., Rothstein A. 1964; Discrimination between alkali metal cations by yeast. I. Effect of pH on uptake. J. gen. Physiol 48:61
    [Google Scholar]
  4. Armstrong W. McD., Rothstein A. 1967; Discrimination between alkali metal cations by yeast. II. Cation interactions in transport. J. gen. Physiol 50:967
    [Google Scholar]
  5. Eddy A. A., Williamson D. H. 1959; Formation of aberrant cell walls and of spores by the growing yeast protoplast. Nature, Lond 183:1101
    [Google Scholar]
  6. Gascon A., Ochoa A. G., Villanueva J. R. 1965; Production of yeast and mould protoplasts by treatment with the strepzyme of Micromonospora AS. Canad. J. Microbiol 11:573
    [Google Scholar]
  7. Hofstee B. H. J. 1952; On the evaluation of the constants Vm and Km in enzyme reactions. Science, N.Y 116:3029
    [Google Scholar]
  8. Indge K. J. 1968; The effects of various anions and cations on the lysis of yeast protoplasts by osmotic shock. J. gen. Microbiol 51:425
    [Google Scholar]
  9. Jeynes M. H. 1961; The growth and division of bacterial protoplasts. Expl Cell Res 24:255
    [Google Scholar]
  10. Lehninger A. L. 1962; Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol. Rev 42:467
    [Google Scholar]
  11. McQuillen K. 1960 In The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. Vol. 1 p. 249 New York and London: Academic Press;
    [Google Scholar]
  12. Shockman G. D., Lampen J. O. 1962; Inhibition by antibiotics of the growth of bacterial and yeast protoplasts. J. Bact 84:508
    [Google Scholar]
  13. Sillén L. G., Martell A. E. 1964; Stability constants of metal-ion complexes. Special Publication, no. 17 London: The Chemical Society;
    [Google Scholar]
  14. Svoboda A., Nečas O. 1966; Regeneration of yeast protoplasts prepared by snail enzyme. Nature, Lond 210:845
    [Google Scholar]
  15. Wachsman J. T., Storck R. 1960; Propionate induced lysis of protoplasts of Bacillus megaterium. J. Bact 80:600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-51-3-433
Loading
/content/journal/micro/10.1099/00221287-51-3-433
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error