SUMMARY: Yeast protoplasts which were in osmotic equilibrium with a 0.55 M-mannitol solution underwent lysis when glucose was added to the suspension. Lysis was dependent on glucose metabolism and was accelerated when chelating agents were added to the medium. Lysis in the presence of glucose and chelating agents was inhibitied by alkali metal ions, magnesium, spermidine and by increasing the tonicity of the test medium. The inhibition of lysis by K and Na when chelating agents were present conformed to a Michaelis-Menten type relationship with apparent dissociation constants of 2–5 × 10–4 M and 3–6 × 10 M for K and Na, respectively.


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