1887

Abstract

SUMMARY

A fertile mutant () isolated from -7 with a mutator gene was found to have a high recipient ability when used as a recipient of the chromosome and F′ and R factors, whereas -7 and -2 , both of which lack the gene, were poor recipients of these foreign deoxyribonucleic acids, -7 exhibited an intermediate recipient ability. These episomal elements, however, were transferred from -7 and -7 to the , and substrains at frequencies comparable to an recipient. In contrast, the frequencies of transfer of these episomes from -7 to the other substrains of were considerably lower than those to -7. The efficiencies of plating () of phage P-22 grown on -7 were likewise lower on -7, -7 and -2 than on -7. The of phage P-22 on -7, -7 and -7 were increased to the value on -7 by its growth on -7 or -2 . The frequencies of transduction of an R factor to these substrains of with phage P-22 grown on various substrains were parallel with the of this phage on these strains. It was further shown that the deoxyribonucleic acid of phage P-22, which had previously been propagated on -7, injected into -7 and -2 is rapidly degraded, whereas appreciable breakdown did not occur in -7. These results with P-22 indicate that -7 is a mutant which is impaired in its capacity of restriction and modification of this phage. The growth of P-22 on -7 resulted in partial increase of its on -7 and -2 , indicating that the restriction and modification capacities of -7 are partially affected. The above results, that -7 acts as a good recipient in the conjugal transfer of chromosome and non-viral episomes and that the frequencies of transfer of the non-viral episomes among the substrains of are comparable to those to -7 and are interpreted as due to the restriction- and modification-less nature of -7.

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1968-02-01
2022-01-25
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