1887

Abstract

SUMMARY: When holotrich ciliates of hay-fed sheep’s rumen acted upon water-soluble carbohydrates under conditions comparable with those in the rumen save for the absence of appreciable bacterial competition, only glucose, fructose, sucrose, inulin, bacterial levan (from ) and to a lesser extent celloblose were utilized for rapid and extensive storage of iodophilic polysaccharide granules (cf. Oxford, 1951). No other soluble carbohydrate tried (including maltose) was so utilized. When polysaccharide storage did take place the product always gave a purple colour with iodine, and had the properties of a starch rather than glycogen in that it gave an insoluble iodine complex under the conditions of Pucher, Leaven-worth & Vickery (1948). Storage of starch by oligotrich ciliates was much slower than with holotrichs, and did not take place with cellobiose as substrate, Holotrich ciliates continued to replace themselves in the sheep's rumen even when large volumes of rumen contents were periodically withdrawn over a long period. The protozoan starch could be isolated in about 1% yield by application of the Pucher (1948) method direct to the dry matter of strained rumen liquor taken from sheep which had grazed on starch-free Spring grass.

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/content/journal/micro/10.1099/00221287-5-4-664
1951-12-01
2021-07-31
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References

  1. Archbold H. K. 1940; Fructosans in the monocotyledons. New Phytol 39:185
    [Google Scholar]
  2. Burroughs W., Frank N. A., Gerlaugh P., Bethke R. M. 1950; Preliminary observations upon factors influencing cellulose digestion by rumen microorganisms. J. Nutrit 40:9
    [Google Scholar]
  3. Doyle W. L. 1943; Nutrition of Protozoa. Biol. Rev 18:119
    [Google Scholar]
  4. Elsden S. R. 1946; The fermentation of carbohydrates in the rumen of sheep. J. exp. Biol 22:51
    [Google Scholar]
  5. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev 14:1
    [Google Scholar]
  6. Kudo R. R. 1947 Protozoology, 3rd ed. Springfield, III: Charles C. Thomas;
    [Google Scholar]
  7. Mangold E. 1929 Handbuch der Ernährung und des Stoffwechsels der Landwirt-schaftlichen Nutztiere Berlin: J. Springer;
    [Google Scholar]
  8. Nielsen J. P. 1943; Rapid determination of starch. An index to maturity in starchy vegetables. Industr. Engng. Chem. Anal. Ed 15:176
    [Google Scholar]
  9. Norman A. G. 1936; The composition of forage crops. 1. Rye grass. Biochem. J 30:1354
    [Google Scholar]
  10. Oxford A. E. 1951; The conversion of certain soluble sugars to a glucosan by holotrich ciliates in the rumen of sheep. J. gen. Micrbbiol 5:83
    [Google Scholar]
  11. Pucher G. W., Leavenworth C. S., Vickery H. B. 1948; Determination of starch in plant tissues. Anal. Chem 20:850
    [Google Scholar]
  12. Tarr H. L. A., Hibbert H. 1931; Formation of dextran by Leuconostoc mesenteroides. Canad. J. Res 5:414
    [Google Scholar]
  13. Trier H. J. 1926; Carbohydrate metabolism of rumen infusoria and the importance of green plant particles for these organisms. Z. vergl. Physiol 4:305
    [Google Scholar]
  14. Wath J. G. van der. 1942; Studies on the alimentary tract of the merino sheep with special reference to the role of the ruminal micro-fauna and flora. Thesis; Univ. of Pretoria:
    [Google Scholar]
  15. Wath J. G. van der. 1948; Studies on the alimentary tract of merino sheep in South Africa. XI. Digestion and synthesis of starch by ruminal bacteria. Onderstepoort J. vet. Sci 23:367
    [Google Scholar]
  16. Wieneck E. 1934; Cellulose digestion in the ciliates of the rumen. Arch. Protistenk 82:169
    [Google Scholar]
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