1887

Abstract

SUMMARY: The growth of bacteria in chemically-defined media containing mixtures of amino-acids is less dependent upon the magnesium concentration than in simple media composed of saits, glucose or glycerol and a single source of nitrogen. In the more complex chemically-defined media growth initially increases rapidly with, and then becomes independent of, the magnesium concentration. Gram-positive bacilli jail to grow when the magnesium content of the medium is less than 0·66 p.p.m., whereas this amount of magnesium is almost sufficient to maintain maximum growth of the Gram-negative bacteria. In addition, a deficiency of magnesium (i.e. c. 1–5 p.p.m.) inhibits the cell division of the Gram-positive rod-shaped organisms, and results in the formation of filamentous cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-5-3-485
1951-08-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/5/3/mic-5-3-485.html?itemId=/content/journal/micro/10.1099/00221287-5-3-485&mimeType=html&fmt=ahah

References

  1. Boyd M. J., Logan M. A., Tytell A. A. 1948; The growth requirements of Cl. perfringens. B.P. 6K J. biol. Chem 174:1013
    [Google Scholar]
  2. Gale E. F. 1947; Nitrogen metabolism. Ann. Rev. Microbiol 1:141
    [Google Scholar]
  3. Gale E. F. 1948; Nitrogen metabolism of Gram-positive bacteria. Bull. Johns Hopk. Univ 83:119–75
    [Google Scholar]
  4. Gale E. F. 1949; The assimilation of amino-acids by bacteria. 8. Trace metals in glutamic acid assimilation and their inactivation by 8-hydroxyquinoline. J. gen. Microbiol 3:369
    [Google Scholar]
  5. Glinka-Tschernorutzky E. 1933; Über den Stickstoffumsatz bei B. mycoides. VI. Mitteilung. Über Ausnutzung verschiedener Stickstoffquellen durch den B. mycoides. Biochem. Z 263:144
    [Google Scholar]
  6. Heigener H. 1935 Porter J. R. Quoted by 1946 Bacterial Chemistry and Physiology p. 681 New York: John Wiley and Sons Incor;
    [Google Scholar]
  7. Henry H., Stacey M. 1946; Histochemistry of the Gram-staining reaction for micro-organisms. Proc. roy. Soc. B 133:391
    [Google Scholar]
  8. Koser S. A., Rettger L. F. 1919; Studies on bacterial nutrition. The utilization of nitrogenous compounds of definite chemical composition. J. infect. Dis 24:301
    [Google Scholar]
  9. Krueger K., Peterson W. H. 1945; The response of Lactobacillus casei and Streptococcus faecalis to Vitamin Bc and thymine. J. biol. Chem 158:145
    [Google Scholar]
  10. Nicholas D. J. D. 1951; The use of Aspergillus niger for the bioassay of Mg, Cu, Zn and Mo available in soils to crop plants. J. hort. Sci in the Press
    [Google Scholar]
  11. Shaffer P. A., Hartman A. F. 1920; The iodometric determination of copper and its use in sugar analysis. J. biol. Chem 45:365
    [Google Scholar]
  12. Stephens D., Hinshelwood C. N. 1949; The conditions for optimal growth rate of Bact. lactis aerogenes. J. chem. Soc p. 2516
    [Google Scholar]
  13. Waring W. S., Werkman C. H. 1942; Growth of bacteria in an iron free medium. Arch. Biochem 1:303
    [Google Scholar]
  14. Webb M. 1948; The influence of magnesium on cell division. 1. The growth of Clostridium welchii in complex media deficient in magnesium. J. gen. Microbiol 2:275
    [Google Scholar]
  15. Webb M. 1949a; The influence of magnesium on cell division. 2. The effect of magnesium on the growth and cell division of various bacterial species in complex media. J. gen. Microbiol 3:410
    [Google Scholar]
  16. Webb M. 1949b; The influence of magnesium on cell division. 3. The effect of magnesium on the growth of bacteria in simple, chemically defined media. J. gen. Microbiol 3:418
    [Google Scholar]
  17. Webb M. 1951; The influence of magnesium on cell division. 4. The specificity of magnesium. J. gen. Microbiol 5:480
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-5-3-485
Loading
/content/journal/micro/10.1099/00221287-5-3-485
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error