SUMMARY: A medium was developed for the more rapid culture of [S]Methionine was utilized preferentially as sole sulphur source from which cysteine/cystine were synthesized. Growth was less on cysteine, negligible on cystine and nil on inorganic sulphite and sulphate. Selenium disulphide, amorphous sulphur, stable colloidal (red) selenium and selenourea were extremely toxic, entering the organism by diffusion. Tellurium and seleno-methionine entered the organism, the latter by active transport, but were not toxic for up to 1 week of incubation although synthesis of [Se]seleno-methionine was demonstrated. Selenite and metallic (black) selenium were not toxic and did not enter the organism. Possible mechanisms of toxicity are discussed.


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