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The carotenoid pigments of Sarcina flava (Staphylococcus afermentans) and S. lutea were extracted, purified and separated by thin-layer chromato-graphy into seven fractions. These fractions were hydrocarbon or polar materials. The two bacteria appeared to synthesize identical pigments which could be complexed in vivo to different proteins or to different amounts of a protein, which would explain the apparent differences in the colour of colonies of the two bacteria when grown on nutrient agar.
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