1887

Abstract

SUMMARY: When Gram-negative cell walls were damaged by lysozyme and ethylene-diaminetetra-acetic acid (EDTA) in tris buffer, tris played an active role through its action as an organic cation. Other organic cations such as aliphatic amines and quaternary ammonium compounds were more effective than tris in modifying the cell wall and making it permeable to other solutes. The most effective organic cations had one C to C alkyl chain. At pH10, EDTA 100/°mUg./ml and -dimethyldodecylamine (DDA) 2.5 °mUg./ml. modified the cell wall of so as to permit a more than 99.99% kill by 20 °mU/g./ml. of a zwitterionic surface active agent, 2-hydroxy-3-(dimethylhexadecylammonio) propane-1-sulphonate (HAPS), in 10 min. at 37°dG. At these concentrations, the individual compounds were bactericidally ineffective. Four other Gram-negative species were shown to be similarly susceptible to killing by this system. Together, EDTA and DDA appeared to remove surface components of the Gram-negative cell wall. Alone, DDA and other organic cations removed somatic antigens from the cell wall.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-48-3-391
1967-09-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/48/3/mic-48-3-391.html?itemId=/content/journal/micro/10.1099/00221287-48-3-391&mimeType=html&fmt=ahah

References

  1. Asbell M. A., Eagon R. G. 1966a; The role of multivalent cations in the organization and structure of bacterial cell walls. Biochem. biophys. Res. Commun. 22:664
    [Google Scholar]
  2. Asbell M. A., Eagon R. G. 1966b; Role of multivalent cations in the organization, structure, and assembly of the cell wall of Pseudomonas aeruginosa. J. Bact. 92:380
    [Google Scholar]
  3. Colobert L. 1958; fitude de la lyse de Salmonelles pathogenes provoquee par le lysozyme, apres delipidation partielle de la paroi exteme. Annls Inst. Pasteur, Paris 95:156
    [Google Scholar]
  4. Gilby A. R., Few A. V. 1960; Lysis of protoplasts of Micrococcus lysodeikticus by ionic detergents. J. gen. Microbiol. 23:19
    [Google Scholar]
  5. Goldschmidt M. C., Wyss O. 1966; Chelation effects on Azotobacter cells and cysts. J. Bact. 91:120
    [Google Scholar]
  6. Gray G. W., Wilkinson S. G. 1965; The effect of ethylenediaminetetraacetic acid on the cell walls of some Gram-negative bacteria. J. gen. Microbiol. 39:385
    [Google Scholar]
  7. Herzberg M., Green J. H. 1964; Composition and characteristics of cell walls of smooth strains of Salmonella typhimurium and derived rough variants. gen. Microbiol. 35:421
    [Google Scholar]
  8. Hotchkiss R. D. 1946; The nature of the bactericidal action of surface active agents. Ann. N.Y. Acad. Sci. 46:479
    [Google Scholar]
  9. Humphrey B., Vincent J. M. 1962; Calcium in cell walls of Rhizobium trifolii. J. gen. Microbiol. 29:557
    [Google Scholar]
  10. Leive L. 1965; Release of lipopolysaccharide by EDTA treatment of Escherichia coli. Biochem. biophys. Res. Commun. 21:290
    [Google Scholar]
  11. Martin H. H. 1963; Bacterial protoplasts—a review. J. theoret. Biol. 5:1
    [Google Scholar]
  12. Nossal N. G., Heppel L. A. 1966; The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J. biol. Chem. 241:3055
    [Google Scholar]
  13. Osawa E., Muschel L. H. 1964; Studies relating to the serum resistance of certain Gram-negative bacteria. J. exp. Med. 119:41
    [Google Scholar]
  14. Repaske R. 1958; Lysis of Gram-negative organisms and the role of Versene. Biochim. biophys. Acta 30:225
    [Google Scholar]
  15. Salton M. R. J. 1951; The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J. gen. Microbiol. 5:391
    [Google Scholar]
  16. Shands J. W. 1965; Localization of somatic antigen on Gram-negative bacteria by electron micro-scopy. J. Bact. 90:266
    [Google Scholar]
  17. Voss J. G. 1963; Effect of inorganic cations on bactericidal activity of anionic surfactants. J. Bact. 86:207
    [Google Scholar]
  18. Voss J. G. 1964; Lysozyme lysis of Gram-negative bacteria without production of spheroplasts. J. gen. Microbiol. 35:313
    [Google Scholar]
  19. Wardlaw A. C. 1963; The complement-dependent bacteriolytic action of normal human serum. II. Cell wall composition of sensitive and resistant strains. Can. J. Microbiol. 9:41
    [Google Scholar]
  20. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b. J. gen. Microbiol. 22:158
    [Google Scholar]
  21. Wolin M. J. 1966; Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bact. 91:1781
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-48-3-391
Loading
/content/journal/micro/10.1099/00221287-48-3-391
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error