1887

Abstract

Surmmary

Forty-one strains representing 21 nomen species of the genus and 2 of the genus were screened for sensitivity to ethylene-diaminetetra-acetic acid (EDTA). Sensitivity of organisms was assessed by determining the release of intracellular solutes and the loss of viability under the action of EDTA. The latter criterion was found to be the more useful for the differentiation of sensitive and resistant organisms. Strains of and possibly were considered as resistant to EDTA. Strains of were clearly differentiated from strains of , which were highly resistant to EDTA. The practical value and possible taxonomic significance of the results are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-47-1-67
1967-04-01
2022-05-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/47/1/mic-47-1-67.html?itemId=/content/journal/micro/10.1099/00221287-47-1-67&mimeType=html&fmt=ahah

References

  1. Asbell M. A., Eagon R. G. 1966; The role of multivalent cations in the organization and structure of bacterial cell walls. Biochem.biophys. Res. Commun. 22:664
    [Google Scholar]
  2. Bergey's Manual of Determinative Bacteriology 1957, 7th ed.. Ed. by Breed R. S., Murray E. G. D., Smith N. Baltimore: Williams and Wilkins Co.;
    [Google Scholar]
  3. Bernheim F. 1963; Factors which affect the size of the organisms and the optical density of suspensions of Pseudomonas aeruginosa and Escherichia coli. J. gen. Microbiol. 30:53
    [Google Scholar]
  4. Brown A. D. 1964; Aspects of bacterial response to the ionic environment. Bact. Rev. 28:296
    [Google Scholar]
  5. Brown M. R. W., Richards R. M. E. 1965; Effect of ethylenediaminetetraacetate on the resistance of Pseudomonas aeruginosa to antibacterial agents. Nature, Lond. 207:1391
    [Google Scholar]
  6. Carson K. J., Eagon R. G. 1966; Lysozyme sensitivity of the cell wall of Pseudomonas aeruginosa: further evidence for the role of the non-peptidoglycan components in cell wall rigidity. Can. J. Microbiol. 12:105
    [Google Scholar]
  7. Colobert L. 1957a; Inactivation par le lysozyme de l’antigSnesomatique de SalmonellespathogdneschaufKes. C.r.hebd. Seanc. Acad. Sci., Paris 244:2863
    [Google Scholar]
  8. Colobert L. 1957b; Destruction par le lysozyme, apresdelipidation de la paroiexterne, de Salmonellespathogfcnes. C.r.hebd. Seanc. Acad. Sci., Paris 245:1674
    [Google Scholar]
  9. Colwell R. R., Citarella R. V., Ryman I. 1965; Deoxyribonucleic acid base composition and Adansonian analysis of heterotrophic, aerobic pseudomonads. J. Bact. 90:1148
    [Google Scholar]
  10. De Ley J. 1964; Pseudomonas and related genera. A. Rev. Microbiol. 18:17
    [Google Scholar]
  11. De Ley,J., Park I. W., Tijtgat R., Van Ermengem J. 1966; DNA homology and taxonomy of Pseudomonas and Xanthomonas. J. gen. Microbiol. 42:43
    [Google Scholar]
  12. Eagon R. G., Carson K. J. 1965; Lysis of cell walls and intact cells of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid and by lysozyme. Can. J. Microbiol. 11:193
    [Google Scholar]
  13. Edwards C. D., Noller E. C. 1964; Effect of lysozyme-potentiating treatments on Escherichia coli cell wall protein. Proc. Okla. Acad. Sci. 44:196
    [Google Scholar]
  14. Gray G. W., Wilkinson S. G. 1965a; The action of ethylenediaminetetra-acetic acid on Pseudomonas aeruginosa. J. appl. Bact. 28:153
    [Google Scholar]
  15. Gray G. W., Wilkinson S. G. 1965b; The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria. J. gen. Microbiol. 39:385
    [Google Scholar]
  16. Hamilton-Miller J. M. T. 1965; Effect of EDTA upon bacterial permeability to benzylpenicillin. Biochem.biophys. Res. Commun. 20:688
    [Google Scholar]
  17. Haynes W. C. 1961; Correct description of Pseudomonas mildenbergii Bergey et al. 1930. J. Bact. 81:385
    [Google Scholar]
  18. Herzberg M., Green J. H. 1964; Composition and characteristics of cell walls of smooth strains of Salmonella typhimurium and derived rough variants. J. gen. Microbiol. 35:421
    [Google Scholar]
  19. Ikari,P. & Hugh R. 1963; Pseudomonas alcaligenesMonias 1928), a polar monotrichous dextrose non-oxidiser. Bact. Proc. p. 41
    [Google Scholar]
  20. Leive L. 1965a; Actinomycin sensitivity in Escherichia coli produced by EDTA. Biochem.biophys. Res. Commun. 18:13
    [Google Scholar]
  21. Leive L. 1965b; A non-specific increase in permeability in Escherichia coli produced by EDTA. Proc. natn.Acad. Sci., U.S.A. 53:745
    [Google Scholar]
  22. Leive L. 1965c; Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem.biophys. Res. Commun. 21:290
    [Google Scholar]
  23. Lysenko O. 1961; Pseudomonas—an attempt at a general classification. J. gen. Microbiol. 25:379
    [Google Scholar]
  24. Macgregor D. R., Elliker P. R. 1958; A comparison of some properties of strains of Pseudomonas aeruginosa sensitive and resistant to quaternary ammonium compounds. Can. J. Microbiol. 4:499
    [Google Scholar]
  25. Macleod R. A. 1965; The question of the existence of specific marine bacteria. Bact. Rev. 29:9
    [Google Scholar]
  26. Malek I., Radochova M., Lysenko O. 1963; Taxonomy of the species Pseudomonas odorans. J. gen. Microbiol. 33:349
    [Google Scholar]
  27. Mandel M. 1966; Deoxyribonucleic acid base composition in the genus Pseudomonas. J. gen. Microbiol. 43:273
    [Google Scholar]
  28. Repaske R. 1956; Lysis of Gram-negative bacteria by lysozyme. Biochim.biophys. Acta 22:189
    [Google Scholar]
  29. Repaske R. 1958; Lysis of Gram-negative organisms and the role of Versene. Biochim.biophys. Acta 30:225
    [Google Scholar]
  30. Rhodes M. E. 1958; The cytology of Pseudomonas spp. as revealed by a silver-plating staining method. J. gen. Microbiol. 18:639
    [Google Scholar]
  31. Rhodes M. E. 1959; The characterization of Pseudomonas fluorescens. J. gen. Microbiol. 21:221
    [Google Scholar]
  32. Rhodes M. E. 1965; Flagellation as a criterion for the classification of bacteria. Bact. Rev. 29:442
    [Google Scholar]
  33. Shively J. M., Hartsell S. E. 1964a; Bacteriolysis of the pseudomonads. I. Agents potentiating lysis. Can. J. Microbiol. 10:905
    [Google Scholar]
  34. Shively J. M., Hartsell S. E. 1964b; Bacteriolysis of the pseudomonads. II. Chemical treatments affecting the lytic response. Can. J. Microbiol. 10:911
    [Google Scholar]
  35. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. gen. Microbiol. 43:159
    [Google Scholar]
  36. Strange R. E. 1964; Effect of magnesium on permeability control in chilled bacteria. Nature, Lond. 203:1304
    [Google Scholar]
  37. Strange R. E., Dark F. A. 1965; ‘Substrate-accelerated death’ of Aerobacteraerogenes. J. gen. Microbiol. 39:215
    [Google Scholar]
  38. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J. appl. Bact. 23:37
    [Google Scholar]
  39. Vincent J. M., Humphrey B. A. 1963; Partition of divalent cations between bacterial wall and cell contents. Nature, Lond. 199:149
    [Google Scholar]
  40. Voss J. G. 1964; Lysozyme lysis of Gram-negative bacteria without production of spheroplasts. J.gen. Microbiol. 35:313
    [Google Scholar]
  41. Wilkinson S. G. 1962 The action of ethylenediaminetetra-acetic acid on Pseudomonas aeruginosa. Thesis University of Hull;
    [Google Scholar]
  42. Wolin M. J. 1966; Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme. J. Bact. 91:1781
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-47-1-67
Loading
/content/journal/micro/10.1099/00221287-47-1-67
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error