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Forty-one strains representing 21 nomen species of the genus Pseudomonas and 2 of the genus Alcaligenes were screened for sensitivity to ethylene-diaminetetra-acetic acid (EDTA). Sensitivity of organisms was assessed by determining the release of intracellular solutes and the loss of viability under the action of EDTA. The latter criterion was found to be the more useful for the differentiation of sensitive and resistant organisms. Strains of Pseudomonas diminuta, P. geniculata, P. iodinum, P. maltophilia, P. pavonacea and possibly P. rubescens were considered as resistant to EDTA. Strains of P. alcaligenes were clearly differentiated from strains of Alcaligenes faecalis, which were highly resistant to EDTA. The practical value and possible taxonomic significance of the results are discussed.
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