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When inoculated with a clone of Rhizobium that produced large pink effective root nodules in virus-free plants, white clover plants infected with clover phyllody virus (CPV) produced mainly small white nodules characteristic of reduced effectiveness in nitrogen fixation. Bacteria from nodules borne on these CPV-infected plants again produced mainly small white nodules in virus-free clover cuttings and seedlings, but did not transmit CPV. Either exposure to the virus, or culture in CPV-infected tissues, seemed to induce a change in the Rhizobium.
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