1887

Abstract

SUMMARY

The effect of various modifying agents has been studied on the synergistic interaction of u.v.- and -radiation in the induction of prototrophic revertants of tryptophan-requiring /r 2. The component of -radiation damage involved in the interaction shows a large effect of oxygen during irradiation (dose modifying factor of up to 3) and is thus more like lethal than mutational damage. The interaction mutants respond to various post-irradiation treatments in the same way as u.v.-induced, but not -induced, mutants, i.e. they are photoreactivable, their frequency is increased by acri-flavine and nutrient broth after irradiation and they are susceptible to mutation frequency decline in minimal medium. It is postulated that damage induced in DNA by -radiation inhibits the repair of premutational lesions induced by u.v. thus increasing the yield of u.v.-induced mutants. The possible nature of the system involved in this repair (which is not that responsible for the excision of thymine dimers from DNA) is discussed.

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/content/journal/micro/10.1099/00221287-46-3-339
1967-03-01
2022-01-27
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References

  1. Alper T. 1963; Effects on irradiated micro-organisms of growth in the presence of acriflavine. Nature, Lond 200:534
    [Google Scholar]
  2. Bridges B. A. 1966; A note on the mechanism of u.v. mutagenesis. Mutation Res 3:273
    [Google Scholar]
  3. Bridges B. A., Munson R. J. 1964; Temperature, time and X-ray mutagenesis in E. coli. Mutation Res 1:362
    [Google Scholar]
  4. Bridges B. A., Munson R. J. 1966; Excision-repair of DNA damage in an auxotrophic strain of Escherichia coli . Biochem. biophys. Res. Commun 22:268
    [Google Scholar]
  5. Davies D. R., Arlett C. F., Munson R. J., Bridges B. A. 1967; Interaction between ultraviolet light and γ-radiation damage in the induction of mutants of Escherichia coli: the response in strains with normal and reduced ability to repair ultraviolet damage.
    [Google Scholar]
  6. Demerec M., Latarjet M. 1946; Mutations in bacteria induced by radiations. Cold Spr. Harb.Symp. quant. Biol 11:38
    [Google Scholar]
  7. Doudney C. O. 1963; Ultraviolet light-induced mutation as an event in the physiology of the bacterial cell. In Repair from Genetic Radiation125 London; Pergamon Press:
    [Google Scholar]
  8. Doudney C. O. 1966; Photoreversal of ultraviolet light induced mutation, lethality and damage to nucleic acid formation in bacteria. Mutation Res 3:280
    [Google Scholar]
  9. Doudney C. O., Haas F. L. 1958; Modification of ultraviolet-induced mutation frequency and survival in bacteria by post-irradiation treatment. Proc natn. Acad. SciU.S.A 44390
    [Google Scholar]
  10. Haynes R. H. 1964; Molecular localization of radiation damage relevant to bacterial inactivation. In Physical Processes in Radiation Biology51 New York: Academic Press;
    [Google Scholar]
  11. Hill R. F. 1965; Ultraviolet induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem. Photobiol 4:563
    [Google Scholar]
  12. Kada T., Brun E., Marcovich H. 1960; Comparaison de 1’induction de mutants prototrophes par les rayons X et U.V. chez Escherichia coli b/r try. Ann. Inst. Pasteur 99:547
    [Google Scholar]
  13. Kneser H. 1965; Dark and K-reactivation in U.V. irradiated Escherichia coli . Z. VererbLehre 97:102
    [Google Scholar]
  14. Kneser H. 1966; Absence of K-reactivation in a recombination deficient mutant of E. coli . Biochem. biophys. Res. Commun 22:383
    [Google Scholar]
  15. Kondo S., Jagger J. 1966; Action spectra for photoreactivation of mutation to prototrophy in strains of Escherichia coli possessing and lacking photoreactivating-enzyme activity. Photochem. Photobiol 5:189
    [Google Scholar]
  16. Kondo S., Kato T. 1966; Action spectra for photoreactivation of killing and mutation to prototrophy in U. V.-sensitive strains of Escherichia coli possessing and lacking photoreactivating enzyme. Photochem. Photobiol in the Press;
    [Google Scholar]
  17. McGrath R. A., Williams R. W. 1966; Evidence for repair in X-irradiated DNA. A bstr.Biophys. Soc73
    [Google Scholar]
  18. Munson R. J., Bridges B. A. 1966; Non-photoreactivating repair of mutational lesions induced by ultraviolet and ionizing radiations in Escherichia coli. Mutation Res 3:461
    [Google Scholar]
  19. Setlow R. B., Carrier W. L. 1964; The disappearance of thymine dimers from DNA: an error correcting mechanism. Proc natn. Acad. SciU.S.A 51226
    [Google Scholar]
  20. Witkin E. M. 1956; Time temperature and protein synthesis: a study of ultraviolet induced mutation in bacteria. Cold Spr. Harb. Symp. quant. Biol 21:123
    [Google Scholar]
  21. Witkin E. M. 1963; The effect of acrifiavine on photoreversal of lethal and mutagenic damage produced in bacteria by ultraviolet light. Proc. natn. Acad. SciU.S.A 50425
    [Google Scholar]
  22. Witkin E. M. 1964; Photoreversal and ‘dark repair’ of mutations to prototrophy induced by ultraviolet light in photoreactivable and non-photoreactivable strains of Escherichia coli . Mutation Res 1:22
    [Google Scholar]
  23. Witkin E. M. 1966; Radiation-induced mutations and their repair. Science 152:1345
    [Google Scholar]
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