1887

Abstract

SUMMARY

The effect of various modifying agents has been studied on the synergistic interaction of u.v.- and -radiation in the induction of prototrophic revertants of tryptophan-requiring /r 2. The component of -radiation damage involved in the interaction shows a large effect of oxygen during irradiation (dose modifying factor of up to 3) and is thus more like lethal than mutational damage. The interaction mutants respond to various post-irradiation treatments in the same way as u.v.-induced, but not -induced, mutants, i.e. they are photoreactivable, their frequency is increased by acri-flavine and nutrient broth after irradiation and they are susceptible to mutation frequency decline in minimal medium. It is postulated that damage induced in DNA by -radiation inhibits the repair of premutational lesions induced by u.v. thus increasing the yield of u.v.-induced mutants. The possible nature of the system involved in this repair (which is not that responsible for the excision of thymine dimers from DNA) is discussed.

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/content/journal/micro/10.1099/00221287-46-3-339
1967-03-01
2024-04-26
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