1887

Abstract

SUMMARY

The aerobic growth of was studied in chemically defined media consisting of mineral salts (ammonium as N source) and various benzenoid compounds singly as sole source of carbon+energy. A strain adapted to a defined glucose mineral salts (ammonium as N source) medium grew readily in concentrations of benzaldehyde, benzoate, -hydroxybenzoate or phenylacetate of the order 10 to 10 . The initial growth rate in benzoate, -hydroxybenzoate or phenylacetate increased and then decreased with increasing concentration of benzenoid compound, according to a relationship which is interpreted by analogy with enzyme inhibition by excess substrate. The kinetics of growth inhibition by the above compounds in glucose salts medium were similar to those in the simplest case of inhibition of an enzyme or heterogeneous catalyst. During repeated subculture in benzoate, -hydroxybenzoate or phenylacetate as sole carbon source, the bacteria showed various adaptive responses which include an immediate decrease in the lag and an increase in the growth rate. As the concentration of benzoate to which the bacteria had become adapted to utilize as sole carbon+energy source was increased, the molar growth yield and the growth rate changed in a related manner and the inhibitory action of benzoate in glucose salts medium decreased.

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/content/journal/micro/10.1099/00221287-46-2-213
1967-02-01
2021-10-20
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References

  1. Albert A. 1965 Selective Toxicity, 3rd. London: Methuen;
    [Google Scholar]
  2. Ashmore P. G. 1963 Catalysis and Inhibition of Chemical Reactions London: Butterworth;
    [Google Scholar]
  3. Baskett A. C., Hinshelwood C. N. 1950; The utilization of carbon sources by Bact. lactis aerogenes. I. General survey. Proc. R. Soc. B 136520
    [Google Scholar]
  4. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. J. gen. Microbiol 23:457
    [Google Scholar]
  5. Cruess W. V., Richert P. M. 1929; Effects of hydrogen ion concentration on the toxicity of sodium benzoate to micro-organisms. J. Bact 17:363
    [Google Scholar]
  6. Dagley S., Dawes E. A., Morrison G. A. 1950; Factors influencing the early phases of growth of Aerobacter aerogenes. J. gen. Microbiol 4:437
    [Google Scholar]
  7. Dean A. C. R., Hinshelwood C. N. 1963; Integration of cell reactions. Nature, Lond 199:7
    [Google Scholar]
  8. Dean A. C. R., Hinshelwood C. N. 1964; Some basic aspects of cell regulation. Nature, Lond 201:232
    [Google Scholar]
  9. Dixon M., Webb E. C. 1958 Enzymes London: Longmans Green;
    [Google Scholar]
  10. Goshorn R. H., Degering E. F. 1938; Preparation of alpha-phenylalkanoic acids and a study of their bactericidal and physical properties. J. Am. pharm. Ass 27:865
    [Google Scholar]
  11. Herbert D. 1958; Some principles of continuous culture. In Recent Progress in Microbiology. Tunevall G. Symp. 1th int. Congr. Microbiol381
    [Google Scholar]
  12. Hinshelwood C. N. 1940 The Kinetics of Chemical Change Oxford: Clarendon Press;
    [Google Scholar]
  13. Hinshelwood C. N. 1946 The Chemical Kinetics of the Bacterial Cell Oxford: Clarendon Press;
    [Google Scholar]
  14. Monod J. 1942 Recherches sur la Croissance des Cultures Bactériennes Paris: Herman et Cie;
    [Google Scholar]
  15. Murray D. R. P. 1930; The inhibition of esterases by excess substrate. Biochem. J 24:1890
    [Google Scholar]
  16. Pirt S. R. 1965; The maintenance energy of bacteria in growing cultures. Proc. R. Soc. B 163224
    [Google Scholar]
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