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SUMMARY: The % survivals of Escherichia coli (B, Jepp, commune), Serratia marcescens (uk8) and Aerobacter aerogenes (Hinshelwood), in the presence of polyethylene glycol were studied as a function of molecular weight and % polyethylene glycol. Polyethylene glycol is shown to cause a loss of viability; experiments are described which suggest loci for its action.