1887

Abstract

SUMMARY: strain B was sensitized to the action of γ-radiation or ultraviolet (u.v.) radiation by incorporating 5-bromouracil into the DNA of the bacteria. Most sensitization was observed after u.v. irradiation, less after anoxic γ-irradiation and least after aerobic γ-irradiation. Incubation of the bacteria for a few hours after irradiation on a nutrient medium which included chloramphenicol generally resulted in extensive restoration of colony-forming ability whether or not the bacteria contained 5-bromouracil. Only after the aerobic γ-irradiation of bacteria containing no bromoruacil was little restoration obtained, After aerobic or anaerobic γ-irradiation the ‘rescue’ of bacteria containing 5-bromouracil was relatively larger than that observed for bacteria containing no bromouracil. Maximum restoration was obtained after u.v. irradiation and this occurred to about the same extent, whether or not bromouracil had been incorporated into the bacteria. The results suggest that treatment with chloramphenicol decreases the expression of radiation-induced lesions which occur in the bacterial DNA; this may account for the mechanism of action of the inhibitor.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-45-1-97
1966-10-01
2021-09-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/45/1/mic-45-1-97.html?itemId=/content/journal/micro/10.1099/00221287-45-1-97&mimeType=html&fmt=ahah

References

  1. Alper T. 1955; Bacteriophage as indicator in radiation chemistry.. Radiat. Res 2:119
    [Google Scholar]
  2. Alper T. 1961; Variability in the oxygen effect observed with microorganisms. II. Escherichia coli b.. Int. J. Radiat. Biol 3:369
    [Google Scholar]
  3. Alper T. 1962; Evidence for two resolvable sites of action of radiation on microorganisms.. Abh. dtsch. Akad. Wiss, Berl., Klasse für Medizin, no. 1153
    [Google Scholar]
  4. Alper T. 1963; Lethal mutations and cell death.. Phys. Med. Biol 8:365
    [Google Scholar]
  5. Alper T., Gillies N. E. 1958; Restoration of Escherichia coli strain b after irradiation; its dependence on suboptimal growth conditions.. J. gen. Microbiol 18:461
    [Google Scholar]
  6. Alper T., Gillies N. E. 1960; The relationship between growth and survival after irradiation of Escherichia coli strain b and two resistant mutants.. J. gen. Microbiol 22:113
    [Google Scholar]
  7. Alper T., Moore J. L. 1964; The interaction of some modifying treatments with the sensitizing action of 5-bromouracil in bacteria. (Abstract). Brit. J. Radiol 37:803
    [Google Scholar]
  8. Boyce R. P., Flanders P. 1964; Release of u.v. light induced thymine dimers from DNA in Escherichia coli k 12.. Proc. natn. Acad. Sci., U.S.A 51:293
    [Google Scholar]
  9. Doudney C. O., Haas F. L. 1958; Modification of ultraviolet-induced mutation frequency and survival of bacteria by post-irradiation treatment.. Proc. natn. Acad. Sci., U.S.A 44:390
    [Google Scholar]
  10. Forage A. J., Gillies N. E. 1964; Restoration of Escherichia coli strain b after γ-irradiation.. J. gen. Microbiol 37:33
    [Google Scholar]
  11. Gillies N. E. 1961; The use of auxotrophic mutants to study restoration in Escherichia coli b after ultraviolet irradiation.. Int. J. Radiat. Biol 3:379
    [Google Scholar]
  12. Gillies N. E., Alper T. 1959; Reduction in the lethal effects of radiation on Escherichia coli b by treatment with chloramphenicol.. Nature, Lond 183:237
    [Google Scholar]
  13. Greer S. 1960; Studies on ultra-violet irradiation of Escherichia coli containing 5-bromouracil in its DNA.. J. gen. Microbiol 22:618
    [Google Scholar]
  14. Howard-Flanders P. 1961; Factors affecting radiation injury to DNA in bacteria and bacteriophage systems.. Fundamental Aspects of Radiosensitivity. Brookhaven Symp. Biol. no. 1418
    [Google Scholar]
  15. Howard-Flanders P., Boyce R. P., Theriot L. 1962; Mechanism of sensitization to ultra-violet fight of T1 bacteriophage by the incorporation of 5-bromodeoxyuridine or by pre-irradiation of the host cell. Nature, Lond 195:51
    [Google Scholar]
  16. Kaplan H. S., Smith K. C., Tomlin P. A. 1962; Effect of halogenated pyrimidines on radiosensitivity of E. coli.. Radiat. Res 16:98
    [Google Scholar]
  17. Kaplan H. S., Zavarine R., Earle J. 1962; Interaction of the oxygen effect and radiosensitization produced by base analogues incorporated into deoxyribonucleic acid.. Nature, Lond 194:662
    [Google Scholar]
  18. Lederberg J. 1950; Isolation and characterisation of biochemical mutants of bacteria.. Meth. med. Res 3:5
    [Google Scholar]
  19. Lett J. T., Parkins G., Alexander P., Ormerod M. G. 1964; Mechanisms of sensitization to X-rays of mammalian cells by 5-bromodeoxyuridine.. Nature, Lond 203:593
    [Google Scholar]
  20. Lorkiewitz Z., Szybalski W. 1960; Genetic effects of halogenated thymidine analogs incorporated during thymidylate synthetase inhibition.. Biochem. biophys. Res. Comm 2:413
    [Google Scholar]
  21. Miller N., Wilkinson J. 1952; Actinometry of ionizing radiations.. Discuss. Faraday Soc 12:50
    [Google Scholar]
  22. Moore P. G., Edwards D. E. 1965Standard Statistical Calculations London: Pitman.;
  23. Sauerbier W. 1961; The influence of 5-bromodeoxyuridine substitution on uv sensitivity, host-cell reactivation, and photoreactivation in T1 and P22H5.. Virology 15:465
    [Google Scholar]
  24. Setlow R. B., Carrier W. L. 1964; The disappearance of thymine dimers from DNA: an error-correcting mechanism.. Proc. natn. Acad. Sci. U.S.A 51:226
    [Google Scholar]
  25. Shugar D. 1965Quoted in Recent Progress in Photobiology86 Oxford: Blackwell.;
  26. Stahl F. W., Crasemann J. M., Okun L., Fox E., Laird C. 1961; Radiation- sensitivity of bacteriophage containing 5-bromodeoxyuridine.. Virology 13:98
    [Google Scholar]
  27. Witkin E. M. 1956; Time, temperature and protein synthesis; a study of ultraviolet-induced mutation in bacteria.. Cold Spr. Harb. Symp. quant. Biol 21:123
    [Google Scholar]
  28. Witkin E. M., Theil E. C. 1960; The effect of post-irradiation treatment with chloramphenicol on various UV-induced mutations in E. coli.. Proc. natn. Acad. Sci. U.S.A 46:226
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-45-1-97
Loading
/content/journal/micro/10.1099/00221287-45-1-97
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error