1887

Abstract

SUMMARY: The results are presented of a survey of NAD and NADP concentration in twenty micro-organisms. They fell into three categories with respect to NAD concentration. (i) High NAD (> 4·5 μmoles/g. dry weight) in obligate anaerobes, members of the Lactobacillaceae and . (ii) Medium NAD (1·0-3·0 μmoles/g. dry weight) in faculative anaerobes, photosynthetic bacteria and . (iii) Low NAD (< 0·9 μmoles/g. dry weight) in obligate aerobes.

Different categories were not found with respect to NADP concentration.

Consistent differences in NAD concentration due to conditions of aeration were not found, but growth on different substrates frequently led to changes in NAD concentration. The concentration of NAD in grown on gluconate was only 6% of the concentration in glucose-grown organisms. In NAD concentrations when grown on formate or oxalate were 13 and 8 times, respectively, greater than the concentration in acetate-grown organisms.

NADP concentrations in and were increased 2- to 5-fold by aeration. In and the concentrations of NAD found after growth in complex media were 3-5 times greater than the amounts found after growth on minimal media. This effect was not observed with .

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1966-08-01
2021-07-26
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References

  1. Barton-Wright E.C. 1946 Practical Methods for the Microbiological Assay of the Vitamin B Complex and Essential Amino Acids. London: Ashe Laboratories.;
    [Google Scholar]
  2. Bassham J. A., Birt L. M., Loening U. E. 1959; Determination of the reduced and oxidized pyridine nucleotides in animal tissues.. Biochem. J. 73:491
    [Google Scholar]
  3. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply.. J. gen. Microbiol. 23:457
    [Google Scholar]
  4. Bonnichsen R. 1962 Methoden der enzymatischen Analyse. Ed. by Bergmeyer H.-U. 285 Weinheim, German Federal Republic: Verlag Chemie.;
    [Google Scholar]
  5. Brock T. D. 1964; Knots in Leucothrix mucor . Science 144:870
    [Google Scholar]
  6. Carnahan, J E., Castle J. E. 1958; Some requirements of biological nitrogen fixation.. J. Bact. 75:121
    [Google Scholar]
  7. Dalziel K. 1962; Kinetic studies of liver alcohol dehydrogenase.. Biochem. J. 84:244
    [Google Scholar]
  8. DeMoss R. D. 1955; Glucose-6-phosphate and 6-phospho-gluconate dehydrogenases from Leuconostoc mesenteroides . Meth. Enzymol. 1:238
    [Google Scholar]
  9. Gibbs M., Sokatch J. T., Gunsalus I. C. 1955; Product labeling of glucose-1-C14 fermentation by homofermentative and heterofermentative lactic acid bacteria.. J. Bact. 70:573
    [Google Scholar]
  10. Greenbaum A. L., Clark J. B., McLean P. 1965; The estimation of the oxidized and reduced forms of the nicotinamide nucleotides.. Biochem. J. 95:161
    [Google Scholar]
  11. Hoare D. S. 1963; The photo-assimilation of acetate by Rhodospirillum rubrum . Biochem. J. 87:284
    [Google Scholar]
  12. Hohorst H.-J. 1962 Methoden der enzymatischen Analyse. Ed. by Bergmeyer H.-U. 134 Weinheim, German Federal Republic: Verlag Chemie.3;
    [Google Scholar]
  13. Holland I. B. 1961; The purification and properties of megacin, a bactenocin from Bacillus megaterium. . Ph.D. Thesis, University of Sheffield.:
    [Google Scholar]
  14. Jensen H. L., Spencer D. 1947; The influence of molybdenum and germanium on nitrogen fixation by Clostridium butyricum. . Proc. Linnean Soc., N.S.W., 72:73
    [Google Scholar]
  15. Johnson P. A., Mortimer M. C., Quayle J. R. 1964; Use of a purified bacterial formate dehydrogenase for the micro-estimation of formate.. Biochim. biophys. Acta, 89:351
    [Google Scholar]
  16. Kaplan N. O. 1960 The Enzymes. Ed. by Boyer P. D., Lardy H., Myrbäck K. 3105 New York, N.Y., U.S.A.: Academic Press Inc.;
    [Google Scholar]
  17. Kemp R. G., Rose I. A. 1964; Coupling of reduced pyridine nucleotide in Leuconostoc mesenteroides. . J. biol. Chem. 239:2998
    [Google Scholar]
  18. Kornberg H. L. 1958; The metabolism of C2 compounds in micro-organisms. 1. The incorporation of [2-14C]-2acetate by Pseudomonas fluorescens and by a Corynebacterium, grown on ammonium acetate.. Biochem. J. 68:535
    [Google Scholar]
  19. Kornberg H. L., Collins J. F., Bigley D. 1960; The influence of growth substrates on metabolic pathways in Micrococcus denitrificans. . Biochim. biophys. Acta 39:9
    [Google Scholar]
  20. Krebs H. A., Bellamy D. 1960; The interconversion of glutamic acid and aspartic acid in respiring tissues.. Biochem. J. 75:523
    [Google Scholar]
  21. Larsen H. 1952; On the culture and general physiology of the green sulfur bacteria.. J. Bact. 64:187
    [Google Scholar]
  22. Lascelles J. 1959; Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: change in activity of enzymes concerned in pyrrole synthesis.. Biochem. J. 72:508
    [Google Scholar]
  23. Lovenberg W., Buchanan B. B., Rabinowitz J. C. 1963; The chemical nature of clostridial ferredoxin.. J. biol. Chem. 238:3899
    [Google Scholar]
  24. Mencher J. M., Heim A. H. 1962; Melanin biosynthesis by Streptomyces lavendulae.. J. gen. Microbiol. 28:665
    [Google Scholar]
  25. P-L Biochemicals 1961 Ultra-Violet Absorption Spectra of Pyridine Nucleotide Coenzymes and Coenzyme Analogues. Milwaukee, Wis., U.S.A.: Pabst Laboratories.;
    [Google Scholar]
  26. Pangborn J., Marr A. G., Robrish S. A. 1962; Localization of respiratory enzymes in intracytoplasmic membranes of Azotobader agilis.. J. Bact. 84:669
    [Google Scholar]
  27. Quayle J. R., Keech D. B., Taylor G. A. 1961; Carbon assimilation by Pseudomonas oxalaticus (OXI) 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalate.. Biochem. J. 78:225
    [Google Scholar]
  28. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli . Publ. Carnegie Instn, no. 607.
    [Google Scholar]
  29. Sokatch J. T., Gunsalus I. C. 1957; Aldonic acid metabolism. 1. Pathway of carbon in an inducible gluconate fermentation by Streptococcus faecalis.. J. Bact. 73:452
    [Google Scholar]
  30. Takebe I., Kitahara K. 1963; Levels of nicotinamide nucleotide coenzymes in lactic acid bacteria.. J. gen. appl. Microbiol. 9:31
    [Google Scholar]
  31. Takebe I, Shirakawa T., Kitahara K. 1964; Quantitative studies of enzyme system of heterolactic fermentation. 1. Some aspects of cytoplasm of Leuconostoc mesenteroides as the site of heterolactic fermentation.. J. gen. appl. Microbiol. 10:359
    [Google Scholar]
  32. Walker D. J. 1958; The purification and properties of the L?##-threonine deaminase of the rumen micro-organism LCI.. Biochem. J. 69:524
    [Google Scholar]
  33. Whitaker A. M. 1961 The growth of micro-organisms in relation to their energy supply. Ph.D. Thesis, University of Sheffield.:
    [Google Scholar]
  34. Wickerham L. J. 1951; Taxonomy of yeasts.. Tech. Bull., U.S. Dept. Agric., no. 1029.
    [Google Scholar]
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