1887

Abstract

SUMMARY: Three strains of penicillin-induced and one strain of D-cycloserine-induced L-forms of were investigated for components of the normal bacterial cell wall. None of the 4 strains was found to contain more than 0.008% of its dry weight as muramic acid, indicating the lack of mucopeptide. These forms also lacked the cell-wall ribitol teichoic acid as shown by the failure to yield any material containing ribitol. Neither muramic acid nor ribitol was detected as acid-soluble nucleotides in the L-forms, and teichoic acid was not released into the supernatant medium. Serological evidence for polysaccharide A was not found, but there was serological identity between extracts of membranes obtained from staphylococci and extracts of membranes isolated from the L-forms.

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/content/journal/micro/10.1099/00221287-42-1-115
1966-01-01
2024-12-01
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References

  1. Armstrong J. J., Baddiley J., Buchanan J. G., Carss B., Greenberg G. R. 1958; Isolation and structure of ribitol phosphate derivatives (teichoic acids) from bacterial walls. J. chem. Soc4344
    [Google Scholar]
  2. Boas N. F. 1953; Method for the determination of hexosamines in tissues. J. biol. Chem 204:553
    [Google Scholar]
  3. Davison A. L., Baddiley J. 1963; The distribution of teichoic acids in staphylococci. J. gen. Microbiol 32:271
    [Google Scholar]
  4. Dienes L., Sharp J. T. 1956; The role of high electrolyte concentration in the production and growth of l-forms of bacteria. J. Bact 71:208
    [Google Scholar]
  5. Dienes L., Weinberger H. J., Madoff S. 1950; Serological reactions of l-type cultures isolated from Proteus. Proc. Soc. exp. Biol. Med 75:409
    [Google Scholar]
  6. Freimer E. H. 1963; Studies of l-forms and protoplasts of group A streptococci. II. Chemical and immunological properties of the cell membrane. J. exp. Med 117:377
    [Google Scholar]
  7. Fuller A. T. 1938; The formamide method for the extraction of polysaccharides from haemolytic streptococci. Br. J. exp. Path 19:130
    [Google Scholar]
  8. Haukenes G., Losnegard N., Oeding P. 1961; Extraction and fractionation of antigenic material from Staphylococcus aureus. Acta path, microbiol. scand 53:84
    [Google Scholar]
  9. Kandler O., Hund A., Zehender C. 1958; Cell wall composition in bacterial and l-forms of Proteus vulgaris. Nature, Lond 181:572
    [Google Scholar]
  10. Klieneberger E. 1942; Some new observations bearing on the nature of the pleuro-pneumonia-like organism known as lI associated with Streptobacillus moniliformis. J. Hyg., Camb. 42 485:
    [Google Scholar]
  11. Marston J. 1961a; Observations on l-forms of staphylococci. J. infect. Dis 108:75
    [Google Scholar]
  12. Marston J. 1961b; Cultivation of staphylococcal l-forms in a liquid medium. J. Bact 81:832
    [Google Scholar]
  13. Morrison T. H., Weibull C. 1962; The occurrence of cell wall constituents in stable Proteus l-forms. Acta path, microbiol. scand 55:475
    [Google Scholar]
  14. Panos C, Barkulis S. S., Hayashi J. A. 1959; Streptococcal l-forms. II. Chemical composition. J. Bact 78:863
    [Google Scholar]
  15. Park J. T., Hancock R. 1960; A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J. gen. Microbiol 22:249
    [Google Scholar]
  16. Perkins H. R., Rogers H. J. 1959; The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus lysodeikticus. Biochem. J 72:647
    [Google Scholar]
  17. Plackett P. 1959; On the probable absence of ‘mucocomplex’ from Mycoplasma mycoides. Biochim. biophys. Acta 35:260
    [Google Scholar]
  18. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  19. Salton M. R. J., Horne R. W. 1951; Bacterial cell wall. II. Methods of preparation and some properties. Biochim. biophys. Acta 45:355
    [Google Scholar]
  20. Schönfeld J. K. 1959; ‘l’-Forms of staphylococci; their reversibility; changes in the sensitivity pattern after several intermediary passages in the ‘l’ phase. Antonie van Leeuwenhoek 25:325
    [Google Scholar]
  21. Schönfeld J. K. 1961; ‘L’-Forms of Staphylococcus. II. Studies on the morphology of the transformation and on the reversibility. Antonie van Leeuwenhoek 27:139
    [Google Scholar]
  22. Sharp J. T. 1963; Amino sugars in L-forms of bacteria and pleuropneumonia-like organisms. J. Bact 86:692
    [Google Scholar]
  23. Sharp J. T., Humans W., Dienes L. 1957; Examination of the L-forms of group A streptococci for the group-specific polysaccharide and M protein. J. exp. Med 105:153
    [Google Scholar]
  24. Smith I. 1960 Chromatographic Techniques I London: Heinemann;
    [Google Scholar]
  25. Williams R. E. O. 1963; L-forms of Staphylococcus aureus. J. gen. Microbiol 33:325
    [Google Scholar]
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