1887

Abstract

SUMMARY:

A system of transformation in is described, in which a wide variety of markers can be transferred from one strain to another. A number of mutants affecting the regulatory, enzymie and serological aspects of penicillinase production are described, classified and discussed. A control gene for penicillinase synthesis was found to be linked to the structural gene in four wild-type strains of The electrophoretic properties of the penicillinases produced by the progeny of inter-strain crosses appeared to be affected by the type of cell in which the relevant structural gene was expressed.

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/content/journal/micro/10.1099/00221287-41-1-7
1965-10-01
2021-10-16
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References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. J. Bact. 81:741
    [Google Scholar]
  2. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315
    [Google Scholar]
  3. Collins J. F. 1964; The distribution and formation of penicillinase in a bacterial population of Bacillus licheniformis. J. gen. Microbiol. 34:363
    [Google Scholar]
  4. Fleming P. C, Goldner M., Glass D. G. 1963; Observations on the nature, distribution and significance of cephalosporinase. Lancet ii:1399
    [Google Scholar]
  5. Gwinn D. D., Thorne C. B. 1964; Transformation of Bacillus licheniformis. J. Bact. 87:519
    [Google Scholar]
  6. Horiuchi T., Novick A. 1961; A thermolabile repression system. Cold Spr. Harb. Symp. quant. Biol. 26:247
    [Google Scholar]
  7. Kogut M., Pollock M. R., Tridgell E. J. 1956; Purification of penicillin-induced penicillinase of Bacillus cereus nrrl 569 : a comparison of its properties with those of a similarly purified penicillinase produced spontaneously by a constitutive mutant strain. Biochem. J. 62:391
    [Google Scholar]
  8. Leonard C. G., Mattheis D. K., Mattheis M. J., Housewright R. D. 1964; Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bact. 88:220
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3:208
    [Google Scholar]
  10. Novick R. P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. J. gen. Microbiol. 33:121
    [Google Scholar]
  11. Perret C. J. 1954; Iodometric assay of penicillinase. Nature, Lond. 174:1012
    [Google Scholar]
  12. Pollock M. R. 1957; Penicillin-induced resistance to penicillin in cultures of Bacillus cereus. Drug Resistance in Micro-organisms78 Ciba Foundation Symposium. Ed. by Wolstenholme G. E. W., O’Connor C. M.
  13. Pollock M. R. 1964; Stimulating and inhibiting antibodies for bacterial penicillinase. Immunology 7:707
    [Google Scholar]
  14. Pollock M. R. 1965; Purification and properties of penicillinases from two strains of Bacillus licheniformis: a chemical, physico-chemical and physiological comparison. Biochem. J. 94:666
    [Google Scholar]
  15. Pollock M. R., Kramer M. 1958; Intermediates in the biosynthesis of bacterial penicillinase. Biochem. J. 70:665
    [Google Scholar]
  16. Pollock M. R., Torriani A. M. 1953; Purification et caractéristiques physicochimiques de la penicillinase de Bacillus cereus. Cr. hebd. Séanc. Acad. Sci., Paris 237:276
    [Google Scholar]
  17. Pollock M. R., Fleming J., Petrie S. 1965; The effects of specific antibodies on the biological activities of wild-type bacterial penicillinases and their mutationally altered analogues. Second Meeting of European Federation of Biochemical SocietiesViennaApril 1965 Report of Colloquium on ‘Antibodies to Biologically Active Molecules’ In the press
    [Google Scholar]
  18. Smithies O. 1955; Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem. J. 61:629
    [Google Scholar]
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