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A system of transformation in Bacillus licheniformis is described, in which a wide variety of markers can be transferred from one strain to another. A number of mutants affecting the regulatory, enzymie and serological aspects of penicillinase production are described, classified and discussed. A control gene for penicillinase synthesis was found to be linked to the structural gene in four wild-type strains of B. licheniformis. The electrophoretic properties of the penicillinases produced by the progeny of inter-strain crosses appeared to be affected by the type of cell in which the relevant structural gene was expressed.
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