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SUMMARY: Nisin, which does not diffuse quickly through agar, may be assayed by dilution methods; or, because it is bactericidal and not merely bacteriostatic, it may be assayed by estimating numbers of surviving bacteria.
Nisin in low sublethal concentration simply prolongs the lag-phase of Streptococcus agalactiae; in higher sublethal concentrations it also induces fast-growing resistant strains. In both cases the delay can be accurately related to nisin concentration, growth being measured in terms of acid production.
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