1887

Abstract

SUMMARY

A comparative survey of amino acid, amino sugar, sugar and lipid components of the cell walls of strains of and was made. The cell walls of and , against which ethylenediaminetetra-acetic acid (EDTA) has a potent bactericidal action, differed from those of the other organisms principally in their sugar components and in their high content of phosphorus. EDTA at alkaline pH selectively solubilized a high proportion of the carbohydrate and phosphorus present, apparently as lipopolysaccharides, in the walls of sensitive organisms. It is suggested that metal cations and lipopolysaccharides in the cell walls of and may be essential to the structural integrity of these organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-39-3-385
1965-06-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/39/3/mic-39-3-385.html?itemId=/content/journal/micro/10.1099/00221287-39-3-385&mimeType=html&fmt=ahah

References

  1. Allen R. J. L. 1940; The estimation of phosphorus. Biochem. J 34:858
    [Google Scholar]
  2. Asselineau J. 1962 Les lipides bactériens Paris: Hermann;
    [Google Scholar]
  3. Brown A. D. 1958; An effect of drying the isolated cell walls of Streptococcus faecalis and a Pseudomonas species, Biochim . biophys. Acta 28:445
    [Google Scholar]
  4. Burton A. J., Carter H. E. 1964; Purification and characterisation of the lipid A component of the lipopolysaccharides from Escherichia coli . Biochemistry 3:411
    [Google Scholar]
  5. Campbell J. J. R., Hogg L. A., Strasdine G. A. 1962; Enzyme distribution in Pseudomonas aeruginosa . J. Bact 83:1155
    [Google Scholar]
  6. Colobert L. 1957a; Inactivation par le lysozyme de l’antigène somatique de Salmonelles pathogènes chauffées. C.r. hebd. Séanc. Acad. Sci Paris: 2442863
    [Google Scholar]
  7. Colobert L. 1957b; Destruction par le lysozyme, après délipidation de la paroi externe, de Salmonelles pathogènes. C.r. hebd. Séanc. Acad. Sci Paris: 2451674
    [Google Scholar]
  8. Colobert L. 1958; Étude de la lyse de Salmonelles pathogènes provoquée par lelysozyme, après délipidation partielle de la paroi externe. Ann. Inst. Pasteur 95:156
    [Google Scholar]
  9. Colobert L., Creach O. 1960; Obtention du substrat du lysozyme contenu dans la paroi ectoplasmique d’Eberthella typhi . Biochim. biophys. Acta 40:167
    [Google Scholar]
  10. Cota-Robles E. H., Marr A. G., Nilson E. H. 1958; Submicroscopic particles in extracts of Azotobacter agilis . J. Bact 75:243
    [Google Scholar]
  11. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. J. biol. Chem 204:983
    [Google Scholar]
  12. Few A. V. 1955; The interaction of polymyxin E with bacterial and other lipids. Biochim. biophys. Acta 16:137
    [Google Scholar]
  13. Fischer F. G., Nebel H. J. 1955; Qualitative and quantitative estimation of glucosamine and galactosamine by the method of paper chromatography. Hoppe-Seyl. Z 302:10
    [Google Scholar]
  14. Gray G. M., MacFarlane M. G. 1958; Separation and composition of the phospholipids of ox heart. Biochem. J 70:409
    [Google Scholar]
  15. Gray G. W., Wilkinson S. G. 1965; The action of ethylenediaminetetra-acetic acid on Pseudomonas aeruginosa . J. appl. Bact 28:153
    [Google Scholar]
  16. Grula E. A., Hartsell S. E. 1957; Lysozyme in the bacteriolysis of Gram-negative bacteria. II. Factors influencing clearing during the Nakamura treatment. Canad. J. Microbiol 3:23
    [Google Scholar]
  17. Hanes C. S. 1929; An application of the method of Hagedorn and Jensen to the determination of larger quantities of reducing sugars. Biochem. J 23:99
    [Google Scholar]
  18. Herzberg M., Green J. H. 1964; Composition and characteristics of cell walls of smooth strains of Salmonella typhimurium and derived rough variants. J. gen. Microbiol 35:421
    [Google Scholar]
  19. Holme T., Malmborg A.-S., Cota-Robles E. 1960; Antigens of spheroplast membrane preparations from Escherichia coli B. Nature, Lond 185:57
    [Google Scholar]
  20. Homma J. Y., Suzuki N., Ito F. 1963; The surface structure of Pseudomonas aeruginosa . J. Immunol 90:819
    [Google Scholar]
  21. Humphrey B., Vincent J. M. 1962; Calcium in cell walls of Rhizobium trifolii . J. gen. Microbiol 29:557
    [Google Scholar]
  22. Jermyn M. A., Isherwood F. A. 1949; Improved separation of sugars on the paper partition chromatogram. Biochem. J 44:402
    [Google Scholar]
  23. Kaneshiro T., Marr A. G. 1962; Phospholipids of Azotobacter agilis, Agrobacterium tumefaciens, and Escherichia coli . J. Lipid Res 3:184
    [Google Scholar]
  24. Kanfer J., Kennedy E. P. 1963; Metabolism and function of bacterial lipids. I. Metabolism of phospholipids in Escherichia coli B. J. biol. Chem 238:2919
    [Google Scholar]
  25. Kates M., Adams G. A., Martin S. M. 1964; Lipids of Serratia marcescens . Canad. J. Biochem 42:461
    [Google Scholar]
  26. Levine C., Chargaff E. 1951; Procedures for the microestimation of nitrogenous phosphatide constituents. J. biol. Chem 192:465
    [Google Scholar]
  27. Lis E. W., Tinocq J., Okey R. 1961; A micromethod for fractionation of lipids by silicic acid chromatography. Analyt. Biochem 2:100
    [Google Scholar]
  28. Mandelstam J. 1962; Preparation and properties of the mucopeptides of cell walls of Gram-negative bacteria. Biochem. J 84:294
    [Google Scholar]
  29. Marinetti G., Stotz E. 1954; Studies on the structure of sphingomyelin. IV. Configuration of the double bond in sphingomyelin and related lipids and a study of their infrared spectra. J. Amer. chem. Soc 76:1374
    [Google Scholar]
  30. Murti C. R. K. 1960; Preparation of bacterial enzymes by controlled lysis. Biochim. biophys. Acta 45:243
    [Google Scholar]
  31. Noller E. C., Hartsell S. E. 1961a; Bacteriolysis of Entervbacteriaceae. I. Lysis by four lytic systems utilising lysozyme. J. Bact 81:482
    [Google Scholar]
  32. Noller E. C., Hartsell S. E. 1961b; Bacteriolysis of Enterobaderiaceae. II. Pre- and co-lytic treatments potentiating the action of lysozyme. J. Bact 81:492
    [Google Scholar]
  33. Norton J. E., Bulmer G. S., Sokatch J. R. 1963; The oxidation of d-alanine by cell membranes of Pseudomonas aeruginosa . Biochim. biophys. Acta 78:136
    [Google Scholar]
  34. O’Leary W. M. 1962; The fatty acids of bacteria. Bact. Rev 26:421
    [Google Scholar]
  35. Rapport M. M., Alonzo N. 1955; Photometric determination of fatty acid ester groups in phospholipides. J. biol. Chem 217:193
    [Google Scholar]
  36. Repaske R. 1956; Lysis of Gram-negative bacteria by lysozyme. Biochim. biophys. Acta 22:189
    [Google Scholar]
  37. Repaske R. 1958; Lysis of Gram-negative organisms and the role of Versene. Biochim. biophys. Acta 30:225
    [Google Scholar]
  38. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviour of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J. Amer. chem. Soc 77:4844
    [Google Scholar]
  39. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  40. Saito K., Akashi S. 1957; Lipide material of Alcaligenes faecalis. I. Studies on the acetone insoluble fraction by paper electrophoresis. J. Biochem Tokyo: 44511
    [Google Scholar]
  41. Salton M. R. J. 1953; Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim. biophys. Acta 10:512
    [Google Scholar]
  42. Salton M. R. J. 1958; The lysis of micro-organisms by lysozyme and related enzymes. J. gen. Microbiol 18:481
    [Google Scholar]
  43. Salton M. R. J. 1960; Studies of the bacterial cell wall. VII. Monosaccharide constituents of the walls of Gram-negative bacteria. Biochim. biophys. Acta 45:364
    [Google Scholar]
  44. Salton M. R. J. 1961; The anatomy of the bacterial surface. Bact. Rev 25:77
    [Google Scholar]
  45. Salton M. R. J., Pavlik J. G. 1960; Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochim. biophys. Acta 39:398
    [Google Scholar]
  46. Silberman R., Gaby W. L. 1961; The uptake of amino acids by lipids of Pseudomonas aeruginosa . J. Lipid Res 2:172
    [Google Scholar]
  47. Sinha D. B., Gaby W. L. 1964; Structural composition of polar lipid-amino acid complex in Pseudomonas aeruginosa . J. biol. Chem 239:3668
    [Google Scholar]
  48. Stacey M., Barker S. A. 1960 Polysaccharides of Micro-organisms62 Oxford University Press;
    [Google Scholar]
  49. Stoffyn P. J., Jeanloz R. W. 1954; Hyaluronic acid and related substances. XII. Identification of amino sugars by paper chromatography. Arch. Biochem 52:373
    [Google Scholar]
  50. Trevelyan W. E., Harrison J. S. 1952; Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem. J 50:298
    [Google Scholar]
  51. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques, 3.274 Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  52. Vincent J. M., Humphrey B. A. 1963; Partition of divalent cations between bacterial wall and cell contents. Nature, Lond 199:149
    [Google Scholar]
  53. Waedlaw A. C. 1963; The complement-dependent bacteriolytic activity of normal human serum. II. Cell wall composition of sensitive and resistant strains. Canad. J. Microbiol 9:41
    [Google Scholar]
  54. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia colt strain B. J. gen. Microbiol 22:158
    [Google Scholar]
  55. Westphal O., Lüderitz O. 1954; Chemische Erforschung von Lipopolysacchariden gramnegativer Bakterien. Angew. Chem 66:407
    [Google Scholar]
  56. Wylie E. B., Johnson M. J. 1962; Effect of penicillin on the cell wall of Escherichia coli . Biochim. biophys. Acta 59:450
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-39-3-385
Loading
/content/journal/micro/10.1099/00221287-39-3-385
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error