1887

Abstract

SUMMARY: Alter ultraviolet irradiation, the growth of (non-lysogenic and lysogenic for phage λ) resulted in diminution of the 8S component of water extracts of the bacteria and the loss of its characteristic spike. In the induced lysogenic bacteria, 8S material was synthesized in the second half of the latent period and the characteristic shape of the boundary was regained. The 8S material was free DNA of relatively high molecular weight and these changes were correlated with alterations in the rate of DNA synthesis in the irradiated bacteria. The synthesis of ribosomal material was found to continue during the development of phage λ. After log phase bacteria had stood for a short time in saline, extracts prepared in tris buffer+0·01 -magnesium acetate showed that an 85S component developed. The latter was replaced by slower material (73S) when the bacteria (non-lysogenic and lysogenic, irradiated or unirradiated) resumed logarithmic growth in broth. This conversion may reflect a configurational change in the 73 S component in environments which do not support growth.

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/content/journal/micro/10.1099/00221287-38-2-231
1965-02-01
2021-10-18
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References

  1. Billen D., Volkin E. 1954; Effect of X-rays on the macro-molecular organisation of Escherichia coli. J. Bact. 67:191
    [Google Scholar]
  2. Brenner S., Jacob F., Meselson M. 1961; An unstable intermediate carrying information from genes to ribosomes for protein synthesis. Nature, Lond. 190:576
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315
    [Google Scholar]
  4. Dagley S., Sykes J. 1956; Studies on an induced enzyme system by means of ultracentrifugal analysis. Arch. Biochem. 62:338
    [Google Scholar]
  5. Dagley S., Sykes J. 1958; The influence of conditions of culture on certain soluble macromolecular components of Escherichia coli. In Microsomal Particles and Protein Synthesis p. 62 Ed. by Roberts R. B. New York: Pergamon Press.;
    [Google Scholar]
  6. Dagley S., Sykes J. 1959; Effect of starvation upon the constitution of bacteria. Nature, Lond. 179:1249
    [Google Scholar]
  7. Doty P., McGill B., Rice S. A. 1958; The properties of sonic fragments of deoxy-ribose nucleic acid. Proc. nat. Acad. Sci., Wash. 44:432
    [Google Scholar]
  8. Fry B. A., Gros F. 1959; The metabolic activities of Escherichia coli during the establishment of lysogeny. J. gen. Microbiol. 21:685
    [Google Scholar]
  9. Gornall A. G., Bardawill C. S., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. J. biol. Chem. 177:751
    [Google Scholar]
  10. Horowitz J., Weller D. 1964; A 30S ribonucleoprotein particle from 50S ribosomes of Escherichia coli. Biochim. biophys. Acta 87:361
    [Google Scholar]
  11. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Br. J. exp. Path. 32:97
    [Google Scholar]
  12. Kelner A. 1953; Growth, respiration and nucleic acid synthesis in ultraviolet irradiated and photo reactivated Escherichia coli. J. Bact. 65:252
    [Google Scholar]
  13. McCarthy B. J. 1960; Variations in bacterial ribosomes. Biochim. biophys. Acta 39:563
    [Google Scholar]
  14. Schachman H. K., Pardee A. B., Stanier R. Y. 1952; Studies on the macro-molecular organisation of microbial cells. Arch. Biochem. Biophys. 38:245
    [Google Scholar]
  15. Schneider W. C. 1945; Phosphorus compounds in animal tissues. 1. Extraction and estimation of deoxypentose nucleic acid and of pentose nucleic acid. J. biol. Chern. 161:293
    [Google Scholar]
  16. Siegel A., Singer S. J., Wildman S. G. 1952; A preliminary study of the high molecular weight components of normal and virus infected Escherichia coli. Arch. Biochem. Biophys. 41:278
    [Google Scholar]
  17. Tissières A., Watson J. D. 1958; Ribonucleoprotein particles from Escherichia coli. Nature, Lond. 182:778
    [Google Scholar]
  18. Tissières A., Watson J. D., Schlessinger D., Hollingworth B. R. 1959; Ribonucleoprotein particles from Escherichia coli. J. molec. Biol. 1:221
    [Google Scholar]
  19. Wade H., Morgan D. 1957; The nature of the fluctuating ribonucleic acid in Escherichia coli. Biochem. J. 65:321
    [Google Scholar]
  20. Waites W. M., Fry B. A. 1964; Effect of infection with phage lambda on the synthesis of protein, RNA and DNA in Escherichia coli. J. gen. Microbiol. 34:413
    [Google Scholar]
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