1887

Abstract

Growth, respiratory activity and the ability to accumulate D-glucosamine were examined in three psychrophilic and three related mesophilic micro-organisms at temperatures between 0° and 20°. Each of the psychrophils (strains of Arthrobacter, Candida and ) grew well at 0° and also respired exogenous glucose and accumulated glucosamine at this temperature. The minimum temperatures for growth (5-10°) of the mesophilic strains of Arthrobacter and were approximately the same as those at which the organisms ceased to respire glucose and accumulate glucosamine. But the mesophil respired exogenous glucose and accumulated glucosamine at temperatures as low as 10°, which is well below the minimum temperature for growth of this bacterium in freshly inoculated culture (20°). Cultures of transferred from 30° to 15° in the mid-exponential phase of growth were capable of a limited amount of growth at the lower temperature, corresponding approximately to a doubling in the size of the population; late-exponential phase cultures when transferred to 15° did not have this ability. Although cultures of transferred from 30° to 10° did not grow, the bacteria were able to respire exogenous glucose and accumulate glucosamine even after 48 hr at the lower temperature. The psychrophilic organisms did not differ from their mesophilic counterparts in their sensitivity to 2,4-dinitrophenol, uranyl ions and nystatin, three metabolic inhibitors which affect respiratory activity and sugar uptake in microorganisms.

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1965-01-01
2024-04-27
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References

  1. Ahmad F., Rose A. H., Garg N. K. 1961; Effect of biotin deficiency on the synthesis of nucleic acids and protein by Saccharomyces cerevisiae. J. gen. Microbiol. 24:69
    [Google Scholar]
  2. Baxter R. M., Gibbons N. E. 1962; Observations on the physiology of psychrophilism in a yeast.. Canad. J. Microbiol. 8:511
    [Google Scholar]
  3. Bergey's Manual of Determinative Bacteriology 1957, 7th ed.. Ed. by Breed R. S., Murray E. G. D., Smith N. R. Baltimore: Williams and Wilkins.;
    [Google Scholar]
  4. Burger M., Hejmova L. 1961; Uptake of metabolizable sugars by Saccharomyces cerevisiae. Folia Microbiol. Delft 6:80
    [Google Scholar]
  5. Cirillo V. P. 1961; Sugar transport in microorganisms.. Annu. Rev. Microbiol. 15:97
    [Google Scholar]
  6. Cirillo V. P., Wilkins P. O., Anton J. 1963; Sugar transport in a psychrophilic yeast.. J. Bad. 86:1259
    [Google Scholar]
  7. Cochrane V. W., Tull D. L. W. 1958; Uranium and spore respiration in Neurospora crassa. Phytopathol. 48:623
    [Google Scholar]
  8. Cohen G. N., Monod J. 1957; Bacterial permeases.. Bad. Rev. 21:169
    [Google Scholar]
  9. Gale E. F. 1954; The accumulation of amino-acids within staphylococcal cells.. Symp. Soc. exp. Biol. 8:242
    [Google Scholar]
  10. Gomori G. 1955; Preparation of buffers for use in enzyme studies.. Meth. Enzymol. 1:138
    [Google Scholar]
  11. Ingraham J. L. 1958; Growth of psychrophilic bacteria.. J. Bad. 76:75
    [Google Scholar]
  12. Ingraham J. L., Bailey G. F. 1959; Comparative study of effect of temperature on metabolism of psychrophilic and mesophilic bacteria.. J. Bad. 77:609
    [Google Scholar]
  13. Kates M., Baxter R. M. 1962; Lipid composition of mesophilic and psychrophilic yeasts (Candida species) as influenced by environmental temperature.. Canad. J. Biochem. Physiol. 40:1213
    [Google Scholar]
  14. Kates M., Hagen P. O. 1964; Influence of temperature on fatty acid composition of psychrophilic and mesophilic Serratia species.. Canad. J. Biochem. Physiol. 42:481
    [Google Scholar]
  15. Kavanatj J. L. 1950; Enzyme kinetics and the rate of biological processes.. J. gen. Physiol. 34:193
    [Google Scholar]
  16. Lampen J. O., Arnow P., Borowska Z., Laskin A. 1962; Location and role of sterol at nystatin-binding sites.. J. Bad. 84:1152
    [Google Scholar]
  17. Linderstrom-Lang K. U., Schellman J. A. 1959 In The Enzymes1 Ed. by Boyer P. D., Lardy H., Myrback K. New York: Academic Press.;
    [Google Scholar]
  18. Maier V. P., Tappel A. L., Volman D. H. 1955; Reversible inactivation of enzymes at low temperatures. Studies of temperature dependence of phosphatase-and peroxidase-catalysed reactions.. J. Am. chem. Soc. 77:1278
    [Google Scholar]
  19. Marini F., Arnow P., Lampen J. O. 1961; The effect of monovalent cations on the inhibition of yeast metabolism by nystatin.. J. gen. Microbiol. 24:51
    [Google Scholar]
  20. Marr A. G., Ingraham J. L., Squires C. L. 1964; Effect of the temperature for growth of Escherichia coli on the formation of β-galactosidase.. J. Bad. 87:356
    [Google Scholar]
  21. Mitchell P. D., Moyle J. M. 1956 In Bacterial Anatomy. Symp. Soc. gen. Microbiol. 6:150
    [Google Scholar]
  22. Ng H., Ingraham J. L., Marr A. G. 1962; Damage and repression in Escherichia coli resulting from growth at low temperatures.. J. Bad. 84:331
    [Google Scholar]
  23. Northam B. E., Norris F. W. 1951; Growth requirements of Schizosaccharomyces odosporus, a yeast exacting towards adenine.. J. gen. Microbiol. 5:502
    [Google Scholar]
  24. Rahn O. 1932 The Physiology of Bacteria. Philadelphia: Blakiston;
    [Google Scholar]
  25. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine.. Biochem. J. 61:586
    [Google Scholar]
  26. Rose A. H. 1963; On the osmotic behaviour of Saccharomyces cerevisiae as affected by biotin deficiency.. J. gen. Microbiol. 31:151
    [Google Scholar]
  27. Rose A. H., Nickerson W. J. 1956; Secretion of nicotinic acid by biotin-dependent yeasts.. J. Bad. 72:324
    [Google Scholar]
  28. Straka R. P., Stokes J. L. 1960; Psychrophilic bacteria from Antarctica.. J. Bad. 80:622
    [Google Scholar]
  29. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques, 3rd ed.. Minneapolis: Burgess Publishing Company;
    [Google Scholar]
  30. Veldkamp H., Van Den Berg G., Zevenhuizen L. P. T. M. 1963; Glutamic acid production by Arthrobader globiformis. Antonie van Leeuwenhoek 29:35
    [Google Scholar]
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