1887

Abstract

Summary: The decay of β-galactosidase activity which occurs when acetone-dried organisms are suspended in water at 25° is prevented by various potassium salts, sodium sulphide, azide, fluoride and citrate, manganous chloride and sulphate, cobaltous chloride, oxine, diethyldithio-carbamate, dimethylglyoxime and 2,4-dinitrophenol. Ethylenediamine-tetra-acetate (EDTA) has no effect on the decay but inhibits the enzyme, the inhibition being annulled by various metal cations, notably manganous and ferrous ions. Sodium pyrophosphate increased β-galactosidase activity of intact organisms. This effect is to some extent reversible and is prevented by glucose. It is not accompanied by uptake of sodium pyrophosphate into the organisms. The possible mode of action of pyrophosphate is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-1-99
1964-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/1/mic-37-1-99.html?itemId=/content/journal/micro/10.1099/00221287-37-1-99&mimeType=html&fmt=ahah

References

  1. Caputto R., Leloir L. F., Trucco R. E. 1948; Lactase and lactose fermentation in Saccharomyces fragilis. Enzymologia 12:350
    [Google Scholar]
  2. Davies A. 1956; Some factors affecting lactase formation and activity in Saccharomyces fragilis. J. gen. Microbiol. 14:425
    [Google Scholar]
  3. Davies R. 1964; Lactose utilization and hydrolysis by Saccharomyces fragilis. J. gen. Microbiol. 37:81
    [Google Scholar]
  4. Davies R., Faulkiner E. A., Wilkinson J. F., Peel J. L. 1951; Ester formation by yeasts. 1. Ethyl acetate formation by Hansenula species. Biochem. J. 49:58
    [Google Scholar]
  5. Epps H. M. R. 1944; Studies on amino-acid decarboxylases. 2. 1( — )-Tyrosine decarboxylase from Streptococcus faecalis. Biochem. J. 38:242
    [Google Scholar]
  6. Lederberg J. 1950; The β-galactosidase of Escherichia coli, strain k-12. J. Bact. 60:381
    [Google Scholar]
  7. Mickle H. 1948; A tissue disintegrator. J. R. micr. Soc. 68:10
    [Google Scholar]
  8. Mohr E., Schramm G. 1960; Reinigung und Charakterisierung der Neuraminidase auf Vibrio cholerae. Z. Natwrf 15B:568
    [Google Scholar]
  9. Oikawa A. 1959; The role of calcium in taka-amylase A. J. Biochem., Tokyo 46:463
    [Google Scholar]
  10. Reithel F. J., Kim J. C. 1960; Studies on the β-galactosidase isolated from Escherichia coliml 308. I. The effect of some ions on enzymic activity. Archiv.Biochem.Biophys. 90:271
    [Google Scholar]
  11. Rickenberg H. V. 1959; The effect of metal ions and proteins on the stability of the β-galactosidase of Escherichia coli. Biochim.biophys. Acta 35:122
    [Google Scholar]
  12. Umbreit W. W., Burris R. H., Stauffer J. F. 1949 Manometric Techniques and Tissue Metabolism, 2nd ed.. Minneapolis: Burgess Publishing Co.;
    [Google Scholar]
  13. Wallenfels K., Malhotra O. P. 1961; Galactosidases. Advanc.Carbohydrate Chem. 16:239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-37-1-99
Loading
/content/journal/micro/10.1099/00221287-37-1-99
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error