1887

Abstract

SUMMARY: Wet and dry Gram staining procedures have different characteristics in respect to their rates of decolorization. It has been proposed that this difference is due to a Gram-positive substrate which dissociates (becomes Gram-negative) in the presence of water. However, the differences between wet and dry Gram procedures can also be explained on the hypothesis that water influences the rate of solvent permeation through cell envelopes. Since no direct proof exists for the reality of the proposed Gram-positive substrate, and since evidence is presented here which cannot be explained by such a hypothesis, it is felt that the solvent permeation concept should be considered seriously as an explanation for the decolorization differences observed between wet and dry Gram procedures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-36-2-257
1964-08-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-36-2-257
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error