1887

Abstract

Summary: Strain of tobacco necrosis virus (TNV) was transmitted by zoospores of 3 different isolates of (Wor.) Dang, to roots of Mung bean and lettuce grown in modified Hoagland’s solution diluted 1/20. On Mung bean roots necrotic local lesions formed one day after exposure to virus and zoospores. Virus in lettuce was assayed by inoculation to leaves of French bean. Virus transmission was favoured by decreasing salt concentration and increasing the pH value of the nutrient solution and depended also on the concentrations of virus and zoospores. With 10zoospores/ml. transmission to lettuce was obtained with as little virus as 0·05 g./l. When the virus concentration was 5 g./l., 50–100 zoospores/ ml. were effective. Fungus infection as measured by the number of zoosporangia in the root was not strictly correlated with virus infection.

Exposure of roots to virus + zoospore mixture for 1 min. sufficed to infect them with virus. More transmission occurred when virus was added before or together with zoospores, than after. Roots, exposed to zoospores for 10 min., then washed, were more readily infected by TNV when virus was introduced during the first hour or two after zoospore attachment to the root cells than later; there was some transmission even when virus was withheld till 4 hr after washing. Immersing roots, inoculated with fungus and virus, in hot water (60°) killed the fungus but not the virus, and varying the interval between inoculation and heating showed that virus became established after 2–3 hr.

Isolates of naturally contaminated by strain d or a of TNV were freed from contamination by inoculating lettuce roots with dilute zoospore suspensions. Zoospores mixed or naturally contaminated with TNV were partially separated from it by centrifugation. Virus transmission was prevented by adding concentrated homologous antiserum to zoospores that had already been exposed to virus, or by adding very dilute antiserum to virus before mixing it with zoospores. The extent to which transmission was prevented by antisera to other strains of TNV depended on the degree of their serological relationship to strain d. The present evidence does not support the suggestion that TNV is carried inside the fungus.

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/content/journal/micro/10.1099/00221287-36-1-79
1964-07-01
2021-07-28
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References

  1. Aknon D. I. 1938; Microelements in culture-solution experiments with higher plants. Am. J. Bot 25:322
    [Google Scholar]
  2. Arnon D. I. 1940; Vitamin B, in relation to the growth of green plants. Science 92:264
    [Google Scholar]
  3. Babos P., Kassanis B. 1963a; Serological relationships and some properties of tobacco necrosis virus strains. J. gen. Microbiol 32:135
    [Google Scholar]
  4. Babos P., Kassanis B. 1963b; The behaviour of some tobacco necrosis virus strains in plants. Virology 20:498
    [Google Scholar]
  5. Badami R. S. 1958; Changes in the transmissibility by aphids of a strain of cucumber mosaic virus. Ann. appl. Biol 46:554
    [Google Scholar]
  6. Bawden F. C., Kassanis B. 1947; Primula obconica , a carrier of tobacco necrosis viruses. Ann. appl. Biol 34:127
    [Google Scholar]
  7. Campbell R. N., Grogan R. G. 1963; Big-vein virus of lettuce and its transmission by Olpidium brassicae. Phytopathology 53:252
    [Google Scholar]
  8. Emerson R. 1958; Mycological organization. Mycologia 50:589
    [Google Scholar]
  9. Hoagland D. R., Snyder W. C. 1933; Nutrition of strawberry plant under controlled conditions: (a) Effects of deficiencies of boron and certain other elements: (6) Susceptibility to injury from sodium salts. Proc. am. Soc. hort. Sci 30:288
    [Google Scholar]
  10. Jacobsen B. 1943; Studies on Olpidium brassicae (Wor.) Dang. Contr. Dep. Plant Path. R. Vet . & Agric. Coll. Copenhagen 24:1
    [Google Scholar]
  11. Sahtiyanci S. 1962; Studien über einige wurzelparasitäre Olpidiaceen. Arch. Mikrobiol 41:187
    [Google Scholar]
  12. Sampson K. 1939; Olpidium brassicae (Wor.) Dang, and its connection with Asterocystis radicis De Wildeman. Trans. Brit, mycol. Soc 23:199
    [Google Scholar]
  13. Smith K. M., Bald J. G. 1935; A description of a necrotic virus disease affecting tobacco and other plants. Parasitology 27:231
    [Google Scholar]
  14. Teakle D. S. 1960; Association of Olpidium brassicae and tobacco necrosis virus. Nature; Lond: 188431
    [Google Scholar]
  15. Teakle D. S. 1962a; Transmission of tobacco necrosis virus by a fungus, Olpidium brassicae. Virology 18:224
    [Google Scholar]
  16. Teakle D. S. 1962b; Necrotic symptoms of tobacco necrosis virus in roots. Phytopathology 52:1037
    [Google Scholar]
  17. Teakle D. S., Gold A. H. 1963; Further studies of Olpidium as a vector of tobacco necrosis virus. Virology 19:310
    [Google Scholar]
  18. Van Der Meer J. H. H. 1926; Rhizoctonia-en Olpidium-aantasting van Bloemkool- planten. Tijdschr. PlZiekt 32:209
    [Google Scholar]
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