1887

Abstract

Summary: The effects of the antifungal polyene antibiotics -acetylcandidin, nystatin and filipin on the yeast cell membranes were compared with the reversibility by K and NH of the inhibition of glycolysis caused by these antibiotics. The results confirm the existence of two functional classes of polyenes which correspond to the number of carbon atoms in the molecule; intermediate types of action can be illustrated within the group of large polyenes. The inhibition of yeast glycolysis by -acetylcandidin, a large polyene, was annulled by K and NH , even at high antibiotic concentrations; inhibition by filipin, a small polyene, was not so annulled. The inhibition of glycolysis by polyenes was associated with the loss of cell K; filipin caused concurrent loss of inorganic phosphate and of accumulated sugar (-sorbose) and initiated ATPase and pyruvate decarboxylase activity. Although -acetylcandidin treatment did not produce loss of accumulated sorbose, added sorbose leaked into treated cells when uptake by way of the hexose transport system had been blocked (by glucose). Thus, definite membrane damage had occurred. Nystatin, a large polyene, was intermediate in effect: at low concentrations its action approached that of -acetylcandidin; at high concentrations, its action was similar to that of filipin. It is suggested that the polyenes present a full spectrum of effects which relate to the degree of physical damage to the cell membrane. They range from filipin which destroys general structural integrity of the membrane even at the minimum concentration necessary to inhibit glycolysis (and growth) to n-acetylcandidin which produces only minimal damage so that relatively specific defects are observed, e.g. those related to K loss and sorbose leakage.

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/content/journal/micro/10.1099/00221287-35-2-249
1964-05-01
2021-10-23
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References

  1. Bolton A. A., Eddy A. A. 1962; The properties of certain particles isolated from yeast protoplasts disrupted by osmotic shock.. Biochem. J. 82:16p
    [Google Scholar]
  2. Cirillo V. P. 1961a; Sugar transport in micro-organisms.. A. Rev. Microbiol. 15:197
    [Google Scholar]
  3. Cirillo V. P. 1961b; The mechanism of sugar transport into the yeast cell.. Trans. N.Y. Acad. Sci., Ser. II 23:725
    [Google Scholar]
  4. Cirillo V. P. 1962; Mechanism of glucose transport across the yeast cell membrane.. J. Bact. 84:485
    [Google Scholar]
  5. Dische Z., Devi A. 1960; Colorimetric method for the determination of ketohexoses in presence of aldoses, ketoheptoses and ketopentoses.. Biochim. biophys. Acta 39:140
    [Google Scholar]
  6. Gottlieb D., Carter H. E., Sloneker J. H., Ammann A. 1958; Protection of fungi against polyene antibiotics by sterols.. Science 128:361
    [Google Scholar]
  7. Gottlieb D., Carter H. E., Sloneker J. H., Wu L.-C., Gaudy E. 1961; Mechanism of inhibition of fungi by filipin.. Phytopathology 51:321
    [Google Scholar]
  8. Harsch M., Lampen J. O. 1963; Modification of K+ transport in yeast by the polyene antifungal antibiotic N-acetylcandidin.. Biochem. Pharmacol. 102:875
    [Google Scholar]
  9. Kinsky S. C. 1962a; Effect of polyene antibiotics on protoplasts of Neurospora crassa.. J. Bact. 83:351
    [Google Scholar]
  10. Kinsky S. C. 1962b; Nystatin binding by protoplasts and particulate fraction of Neurospora crassa, and a basis for the selective toxicity of polyene antifungal antibiotics.. Proc. nat. Acad. Sci., Wash. 48:1049
    [Google Scholar]
  11. Kinsky S. C., Avruch J., Permutt M., Rogers H. B., Schonder A. A. 1962; The lytic effect of polyene antifungal antibiotics on mammalian erythrocytes.. Biochem. biophys. Res. Commun. 9:503
    [Google Scholar]
  12. Lampen J. O. 1962; Intermediary metabolism of fungi as revealed by drug reaction. In Fungi and fungus diseases. Symp. II, N.Y.. Acad. Med., Sec. Microbiol.102 Springfield, 111. U.S.A.: Charles C. Thomas;
    [Google Scholar]
  13. Lampen J. O., Arnow P. M. 1963; Differences in action of large and small polyene antifungal antibiotics.. Bull. Res. Counc. Israel 11, A4:286
    [Google Scholar]
  14. Lampen J. O., Arnow P. M., Borowska Z., Laskin A. I. 1962; Location and role of sterol at nystatin-binding sites.. J. Bact. 84:1152
    [Google Scholar]
  15. Lampen J. O., Arnow P. M., Safferman R. S. 1960; Mechanism of protection by sterols against polyene antibiotics.. J. Bact. 80:200
    [Google Scholar]
  16. Larsen W. G., Demis D. J. 1963; Metabolic studies of the effect of griseofulvin and candicidin on fungi.. J. invest. Derm. 41:335
    [Google Scholar]
  17. Marini F., Arnow P. M., Lampen J. O. 1961; The effect of monovalent cations on the inhibition of yeast metabolism by nystatin.. J. gen. Microbiol. 24:51
    [Google Scholar]
  18. Pullman M. E., Monroy G. C. 1963; A naturally occurring inhibitor of mitochondrial ATPase.. J. biol. Chem. 238:3762
    [Google Scholar]
  19. Stachiewicz E., Quastel J. H. 1963; Amino acid transport in yeast and effects of nystatin.. Canad. J. Biochem. 41:397
    [Google Scholar]
  20. Sutton D. D., Arnow P. M., Lampen J. O. 1961; Effect of high concentrations of nystatin upon glycolysis and cellular permeability in yeast.. Proc. Soc. exp. Biol., N.Y. 108:170
    [Google Scholar]
  21. Umbreit W. W., Burris R. H., Stauffer J. F. 1957; Manometric Techniques, 3rd ed. Minneapolis, Minn., U.S.A.: Burgess Publ. Co..
    [Google Scholar]
  22. Wickerham L. J. 1951; Taxonomy of yeasts.. Tech. Bull. U.S. Dept. Agric.1029
    [Google Scholar]
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