1887

Abstract

Summary: A polarographic technique and Starkey's alkali method have been applied to the qualitative analysis of thiosulphate, trithionate and pentathionate and to the quantitative analysis of tetrathionate in filtrates of bacterial cultures grown in an inorganic medium containing sodium thiosulphate. Five strains of autotrophic thiobacilli and one strain of a heterotrophic bacterium produced polythionates. During thiosulphate oxidation by two autotrophic strains which resembled , tetrathionate, small amounts of trithionate, pentathionate and abundant elemental sulphur were produced and the pH fell from . 7.0 to . 3.0. In cultures of one of the three autotrophic strains which resembled , about half as much tetrathionate, with even smaller amounts of trithionate and pentathionate, was found; abundant sulphur was precipitated and the pH fell to . 4.0. In cultures of the two other strains resembling , small amounts of tetrathionate were detected only occasionally; trithionate and pentathionate were not found, sulphur was deposited and the pH fell to . 4.5. Much tetrathionate, as well as trithionate and pentathionate, were all readily identified in cultures of the heterotrophic organism; the pH rose to . 8.7 and no sulphur was formed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-3-427
1964-03-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/3/mic-34-3-427.html?itemId=/content/journal/micro/10.1099/00221287-34-3-427&mimeType=html&fmt=ahah

References

  1. Baalsrud K. 1954; Some aspects of the physiology of thiobacilli. Autotrophic Micro-organisms. Symp. Soc. gen. Microbiol 4:54
    [Google Scholar]
  2. Baalsrud K., Baalsrud K. S. 1954; Studies on Thiobacillus denitrificans . Arch. Mikrobiol 20:34
    [Google Scholar]
  3. Beijerinck M. W. 1904; Über die Bakterien, welchesich im Dunkelnmit Kohlensäure als Kohlenstoffquelle ernähren können. Zbl. Bakt. (2 Abt. Orig.) 11:593
    [Google Scholar]
  4. Bergey’s Manual of Determinative Bacteriology 1957 7th edition. Ed. by Breed R. S., Murray E. G. D., Smith N. R. London: Ballière, Tindall and Cox Ltd.;
  5. Brezina M., Zuman P. 1958 Polarography in Medicine, Biochemistry and Pharmacy New York: Interscience Publishers Inc;
    [Google Scholar]
  6. Furness W. 1950; The analysis of commercial dithionates and related compounds by polarographic methods. J. Soc. Dy. Col 66:270
    [Google Scholar]
  7. Furness W., Davies W. C. 1952; Polarography of the tetrathionate ion. Analyst 77:697
    [Google Scholar]
  8. Happold F. C., Johnstone K. I., Rogers H. J., Youatt J. B. 1954; Isolation and characteristics of an organism oxidizing thiocyanate. J. gen. Microbiol 10:261
    [Google Scholar]
  9. Happold F. C., Jones G. L., Pratt D. B. 1958; Utilization of thiocyanate by Thiobacillus thioparus and T. thiocyanoxidans . Nature, Lond 182:266
    [Google Scholar]
  10. Johnstone K. I., Townsend M., White D. 1961; Inter-species change in thiobacilli. J. gen. Microbiol 24:201
    [Google Scholar]
  11. Jones G. L., Happold F. C. 1961; The occurrence of polythionates as intermediates in the metabolism of thiosulphate by the thiobacilli. J. gen. Microbiol 26:361
    [Google Scholar]
  12. Kolthoff I. M., Lingane J. J. 1952 Polarography 1 and 2 New York: Interscience Publishers Inc:
    [Google Scholar]
  13. Lees H. 1955 Biochemistry of Autotrophic Bacteria London: Butterworths Scientific Publications;
    [Google Scholar]
  14. Lipman J. G., Waksman S. A., Joffe J. S. 1921; The oxidation of sulphur by soil micro-organisms. Soil Sci 12:475
    [Google Scholar]
  15. Meites L. 1955 Polarographic Techniques New York: Interscience Publishers Inc;
    [Google Scholar]
  16. Milner G. W. C. 1957 The Principles and Applications of Polarography and other Electroanalytical Processes London: Longmans, Green and Co. Ltd;
    [Google Scholar]
  17. Nathansohn A. 1902; Über eine neue Gruppe von Schwefelbakterien und ihren Stoff- wechsel. Mitt. zool. Sta. Neapel 15:655
    [Google Scholar]
  18. Parker C. D. 1945; The corrosion of concrete. I. The isolation of a species of bacterium associated with the corrosion of concrete exposed to atmospheres containing hydrogen sulphide. Aust. J. exp. Biol. med. Sci 23:81
    [Google Scholar]
  19. Parker C. D. 1947; Species of sulphur bacteria associated with the corrosion of concrete. Nature, Lond 159:439
    [Google Scholar]
  20. Parker C. D., Prisk J. 1953; The oxidation of inorganic compounds of sulphur by various sulphur bacteria. J. gen. Microbiol 8:344
    [Google Scholar]
  21. Peck H. D. 1960; Adenosine 5´-phosphosulphate as an intermediate in the oxidation of thiosulphate by Thiobacillus thioparus . Proc. nat. Acad. Sci., Wash 46:1053
    [Google Scholar]
  22. Peck H. D. 1962; Symposium on metabolism of inorganic compounds. V. Comparative metabolism of inorganic sulphur compounds in micro-organisms. Bact. Rev 26:67
    [Google Scholar]
  23. Peck H. D., Fisher E. 1962; The oxidation of thiosulphate and phosphorylation in extracts of Thiobacillus thioparus . J. biol. Chem 237:190
    [Google Scholar]
  24. Pratt D. B. 1958; Detection of polythionate in cultures of thiobacilli by means of the Folin–Ciocalteu reagent. Nature, Lond 181:1075
    [Google Scholar]
  25. Santer M., Margulies M., Klinman N., Kaback R. 1960; Role of inorganic phosphate in thiosulphate metabolism by Thiobacillus thioparus . J. Bact 79:313
    [Google Scholar]
  26. Skarżyński B., Szczepkowski T. W. 1959; Oxidation of thiosulphate by Thiobacillus thioparus . Nature, Lond 183:1413
    [Google Scholar]
  27. Skarżyński B., Ostrowski W. 1958; Incorporation of radioactive sulphur by Thiobacillus thioparus . Nature, Lond 182:933
    [Google Scholar]
  28. Starkey R. L. 1934a; Cultivation of organisms concerned in the oxidation of thiosulphate. J. Bact 28:365
    [Google Scholar]
  29. Starkey R. L. (1934b); The production of polythionates from thiosulphate by micro-organisms. J. Bact 28:387
    [Google Scholar]
  30. Starkey R. L. 1935a; Products of the oxidation of thiosulphate by bacteria in mineral media. J. gen. Physiol 18:325
    [Google Scholar]
  31. Starkey R. L. 1935b; Isolation of some bacteria which oxidize thiosulphate. Soil Sci 39:197
    [Google Scholar]
  32. Starkey R. L. 1956; Transformations of sulphur by micro-organisms. Industr. Engng Chem 48:1429
    [Google Scholar]
  33. Tamiya H., Haga K., Huzisige H. 1941; Zur Physiologie der chemoautotrophen Schwefelbakterien. Acta phytochim. Tokyo 12173 (Quoted by Vishniac & Santer, 1957.):
    [Google Scholar]
  34. Trautwein K. 1921; Beitrag zur Physiologie und Morphologie der Thionsäurebakterien (Omelianski). Zbl. Bakt. ((2. Abt. Orig.) 53:513
    [Google Scholar]
  35. Trudinger P. A. 1959; Initial products of thiosulphate oxidation by Thiobacillus X. Biochim. biophys. Acta 31:270
    [Google Scholar]
  36. Trudinger P. A. 1961a; Thiosulphate oxidation and cytochromes in Thiobacillus X.I. Fractionation of bacterial extracts and properties of cytochromes. Bioehem. J 78:673
    [Google Scholar]
  37. Trudinger P. A. 1961b; Thiosulphate oxidation and cytochromes in Thiobacillus X.II. Thiosulphate-oxidizing enzyme. Bioehem. J 78:680
    [Google Scholar]
  38. Vishniac W. 1952; The metabolism of Thiobacillus thioparus. I. The oxidation of thiosulphate. J. Bact 64:363
    [Google Scholar]
  39. Vishniac W., Santer M. 1957; The Thiobacilli. Bact. Rev 21:195
    [Google Scholar]
  40. Vishniac W., Trudinger P. A. 1962; Symposium on autotrophy. V. Carbon dioxide fixation and substrate oxidation in the chemosynthetic sulphur and hydrogen bacteria. Bact. Rev 26:168
    [Google Scholar]
  41. Waksman S. A. 1922; Micro-organisms concerned in the oxidation of sulphur in the soil. I. Introductory. J. Bact 7:231
    [Google Scholar]
  42. Waksman S. A., Joffe J. S. 1922; Micro-organisms concerned in the oxidation of sulphur in the soil. II. Thiobacillus thiooxidans, a new sulphur-oxidizing organism isolated from the soil. J. Bact 7:239
    [Google Scholar]
  43. Waksman S. A., Starkey R. L. 1923; On the growth and respiration of sulphur-oxidizing bacteria. J. gen. Physiol 5:285
    [Google Scholar]
  44. Woodlley D., Jones G. L., Hapfold F. C. 1962; Some metabolic differences between Thiobacillusthioparus T. denitrificans and T.. J. gen. Microbiol 29:311
    [Google Scholar]
  45. Youatt J. B. 1954; Studies on the metabolism of Thiobacillus thiocyanoxidans . J. gen. Microbiol 11:139
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-34-3-427
Loading
/content/journal/micro/10.1099/00221287-34-3-427
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error