1887

Abstract

Summary: A microphotometric technique is described by which the penicillinase content of a single bacterium in a stable microdrop is measured. The microdrop contains a buffer, benzylpenicillin and bromocresol purple. As the penicillinase hydrolyses the penicillin to penicilloic acid, the concentration of the cationic (purple) form of bromocresol purple decreases. This decrease is measured by a specially designed single-beam microphotometer, and, from the change in light transmission and the volume of the drop, the total penicillinase content of the drop is calculated. The micro-photometer and the calibration of the assay with soluble penicillinase preparations are described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-3-353
1964-03-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/3/mic-34-3-353.html?itemId=/content/journal/micro/10.1099/00221287-34-3-353&mimeType=html&fmt=ahah

References

  1. Alexander J. T., Nastuk W. L. 1953; An instrument for the production of microelectrodes used in electrophysiological studies. Rev. sci. Instrum 24:528
    [Google Scholar]
  2. Collins J. F. 1964; The distribution and formation of penicillinase in a bacterial population of Bacillus licheniformis . J. gen. Microbiol 34:363
    [Google Scholar]
  3. Kogut M., Pollock M. R., Tridgell E. J. 1956; Purification of penicillin-induced penicillinase of Bacillus cereus NRRL 569: a comparison of its properties with those of a similarly purified penicillinase produced spontaneously by a constitutive mutant strain. Biochem. J 62:391
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-34-3-353
Loading
/content/journal/micro/10.1099/00221287-34-3-353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error