1887

Abstract

SUMMARY: A newly isolated strain (c) of is described. The organism oxidizes thiosulphate, sulphide, tetrathionate or trithionate to sulphate; carbon dioxide fixation is coupled to the oxidation of each of these compounds. Concentrations of arsenate, 2,4-dinitrophenol, and other inhibitors of oxidative phosphorylation, inhibit carbon dioxide fixation without appreciably affecting oxygen uptake. Carbon dioxide fixation coupled to sulphide oxidation is more sensitive to arsenate and 2,4-dinitrophenol inhibition than that coupled to thiosulphate oxidation. This result is consistent with the suggestion that thiosulphate oxidation is linked to a substrate phosphorylation which is relatively insensitive to inhibition by 2,4-dinitrophenol and arsenate.

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/content/journal/micro/10.1099/00221287-34-2-307
1964-02-01
2024-12-07
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References

  1. Aubert J-P, Milhaud G., Millet J. 1957; L’assimilation de l’anhydride carbonique par les bactéries chimioautotrophes. Annls. Inst. Pasteur, Paris 92:515
    [Google Scholar]
  2. Clowes G. H. A., Keltch A. K., Strittmatter C. F., Walters C. P. 1950; Action of nitro- and halophenols upon oxygen consumption and phosphorylation by a cell free particulate system from Arbacia eggs. J. gen. Physiol 33:555
    [Google Scholar]
  3. Crane R. K., Lipmann F. 1953; The effect of arsenate on aerobic phosphorylation. J. biol. Chem 201:235
    [Google Scholar]
  4. Cross R. J., Taggart J. V., Covo G. A., Green D. E. 1949; Studies on the cyclo- phorase system. VI. The coupling between oxidation and phosphorylation. J. biol. Chem 177:655
    [Google Scholar]
  5. Gleen H., Quastel J. H. 1953; Sulphur metabolism in soil. Appl. Microbiol 1:70
    [Google Scholar]
  6. Hackett D. P. 1960; Respiratory inhibitors. Encyclopaedia of Plant Physiology (Ed. W. Ruhland); 12, part 223
    [Google Scholar]
  7. Iwatsuka H., Kuno M., Maruyama M. 1962; Studies on the metabolism of a sulphur- oxidizing bacterium. II. The system of CO2-fixation in Thiobacillus thiooxidans . Plant Cell Physiol 3:157
    [Google Scholar]
  8. Jones G. L., Happold F. C. 1961; The occurrence of polythionates as intermediates in the metabolism of thiosulphate by the thiobacilli. J. gen. Microbiol 26:361
    [Google Scholar]
  9. Kelly D. P., Syrett P. J. 1963; Effect of 2:4-dinitrophenol on carbon dioxide fixation by a Thiobacillus. Nature, Lond 197:1087
    [Google Scholar]
  10. Krahl M. E., Clowes G. H. A. 1936; Studies on cell metabolism and cell division. J. gen. Physiol 20:173
    [Google Scholar]
  11. Milhaud G., Millet J. 1957; Synthése de l’adenosine-5′-triphosphate couplée à l’oxydation du thiosulfate par la bactérie chimioautotrophe Thiobacillus denitrificans . C.R. Acad. Sci., Paris 244:1289
    [Google Scholar]
  12. Peck H. D. Jr 1960; Adenosine-5′-phosphosulfate as an intermediate in the oxidation of thiosulfate by Thiobacillus thioparus . Proc. nat. Acad. Sci., Wash 46:1053
    [Google Scholar]
  13. Peck H. D. Jr 1962; Comparative metabolism of inorganic sulfur compounds in microorganisms. Bact. Rev 26:67
    [Google Scholar]
  14. Peck H. D., Fisher E. Jr 1962; The oxidation of thiosulfate and phosphorylation in extracts of Thiobacillus thioparus . J. biol. Chem 237:190
    [Google Scholar]
  15. Pollard F. H. 1954; Inorganic chromatography. Bri. med. Bull 10:187
    [Google Scholar]
  16. Racker E. 1955; Synthesis of carbohydrates from carbon dioxide and hydrogen in a cell free system. Nature, Lond 175:249
    [Google Scholar]
  17. Robbins P. W., Lipmann F. 1958; Separation of the two enzymatic phases in active sulfate synthesis. J. biol. Chem 233:681
    [Google Scholar]
  18. Skarżyński B., Szczepkowski T. W. 1959; Oxidation of thiosulphate by Thiobacillus thioparus . Nature, Lond 183:1413
    [Google Scholar]
  19. Suzuki I., Werkman C. H. 1958; Chemoautotrophic carbon dioxide fixation by extracts of Thiobacillus thiooxidans. I. Formation of oxalacetic acid. Archs. Biochem 76:103
    [Google Scholar]
  20. Trudinger P. A. 1956; Fixation of carbon dioxide by extracts of the strict autotroph Thiobacillus denitrificans . Biochem. J 64:274
    [Google Scholar]
  21. Trudinger P. A. 1959; The initial products of thiosulphate oxidation by Thiobacillus X. Biochim. biophys. Acta 31:270
    [Google Scholar]
  22. Vishniac W. 1952; The metabolism of Thiobacillus thioparus. I. The oxidation of thiosulfate. J. Bact 64:363
    [Google Scholar]
  23. Vishniac W., Santer M. 1957; The Thiobacilli. Bact. Rev 21:195
    [Google Scholar]
  24. Vishniac W., Trudinger P. A. 1962; Carbon dioxide fixation and substrate oxidation in the chemoautotrophic sulfur and hydrogen bacteria. Bact. Rev 26:168
    [Google Scholar]
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