1887

Abstract

SUMMARY: Isoleucine increased the quantity of α-acetolactic acid-forming enzyme which takes part in the synthesis of valine and isoleucine in . Threonine and α-ketobutyric acid, the precursors of isoleucine, had a similar effect. In during the initial stage of growth after inoculation, a temporary substantial increase of α-acetolactic acid-forming enzyme can be observed. This increase was not so great when valine was present, and was enhanced by isoleucine, which was effective during the whole period of growth. The repressive effect of valine can be counteracted with isoleucine and the end product inductive effect of isoleucine can be inhibited by valine. In extracts of disrupted organisms the activity of the α,β-dihydroxy acid dehydrase changes parallel with the concentration of the α-acetolactic acid-forming enzyme.

In the case of the repressive effect of valine on the α-acetolactic acid-forming enzyme can be counteracted with isoleucine.

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/content/journal/micro/10.1099/00221287-34-2-241
1964-02-01
2024-12-08
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References

  1. Cavallini D., Frontali N. 1954; Quantitative determination of keto-acids by paper chromatography. Biochim. biophys. Acta 13:439
    [Google Scholar]
  2. Chibata J., Kisumi M., Ashikaga Y. 1960; Studies on the fermentative production of L-isoleucine. J. biochem. microbiol. Technol. Engng 2:361
    [Google Scholar]
  3. Cohen G. N., Rickenberg H. V. 1956; Concentration spécifiqueréversible des amino acides chez Escherichia coli . Annls. Inst. Pasteur, Paris 91:693
    [Google Scholar]
  4. Davis B. D., Mingioli E. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bact 60:17
    [Google Scholar]
  5. Freundlich M., Burns R. O., Umbarger H.E. 1962; Control of isoleucine, valine and leucine biosynthesis. I. Multivalent repression. Proc. nat. Acad. Sci., Wash 48:1804
    [Google Scholar]
  6. Gorini L. 1960; Antagonism between substrate and repressor in controlling the formation of a biosynthetic enzyme. Proc. nat. Acad. Sci., Wash 46:682
    [Google Scholar]
  7. Gorini L., Gundersen W. 1961; Induction by arginine of enzymes of arginine biosynthesis in E. coli B. Proc. nat. Acad. Sci., Wash 47:961
    [Google Scholar]
  8. Halpern Y. S., Umbarger H. E. 1959; Evidence for two distinct enzyme systems forming acetolactate in Aerobacteraerogenes . J. biol. Chem 234:3067
    [Google Scholar]
  9. Horváth I., Szentirmai A. 1961; Possible causes for production of isoleucine by Streptomyces rimosus . Nature, Lond 192:641
    [Google Scholar]
  10. Horváth I., Szentirmai A. 1962; Valine and isoleucine metabolism of Streptomyces rimosus . Actamicrobiol. hung 9:11
    [Google Scholar]
  11. Leavitt R. I., Umbarger H. E. 1962; Isoleucine and valine metabolism in E. coli. XI. Valine inhibition of the growth of E. coli strain k-12. J. Bact 83:624
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. I., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  13. Meister A., Tice S. V. 1950; Transamination from glutamine toketo acids. J. biol. Chem 187:173
    [Google Scholar]
  14. Radhakrishnan A. N., Snell E. E. 1960; Biosynthesis of valine and isoleucine. II. Formation of α-acetolactate and α-aceto-β-hydroxybutyrate in Neurosporacrassa and E. coli . J. biol. Chem 235:2316
    [Google Scholar]
  15. Sjolander I. R., Folkers K., Adelberg E. A., Tatum E. L. 1954; α, β-dihydroxy-isovaleric acid and α, β-dihydroxy-β-methyl-valeric acid, precursors of valine and isoleucine. J. Am. chem. Soc 76:1085
    [Google Scholar]
  16. Strassmann M., Shatton I. B., Weinhouse S. 1960; Conversion of α-acetolacticacid to the valine precursor, α, β-dihydroxyisovaleric acid. J. biol. Chem 235:700
    [Google Scholar]
  17. Szilárd L. 1960; The control of the formation of specific protein in bacteria and in animal cells. Proc. nat. Acad. Sci., Wash 46:277
    [Google Scholar]
  18. Tatum E. L. 1946; Biochemical mutations in bacteria. Cold Spr. Harb. Symp. quant. Biol 11:278
    [Google Scholar]
  19. Umbarger H. E., Brown B. 1955; Isoleucine and valine metabolism in E. coli. VI. Antagonism between isoleucine and valine. J. Bact 73:105
    [Google Scholar]
  20. Umbarger H. E., Brown B. 1958a; Isoleucine and valine metabolism in E. coli. VII. A negative feedback mechanism controlling isoleucine biosynthesis. J. biol. Chem 233:415
    [Google Scholar]
  21. Umbarger H. E., Brown B. 1958b; Isoleucine and valine metabolism in E. coli. VIII. The formation of acetolactate. J. biol. Chem 233:1156
    [Google Scholar]
  22. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin. J. biol. Chem 161:485
    [Google Scholar]
  23. Wixom R. L., Shatton I. B., Strassmann M. 1960; Studies on a dehydrase in valine biosynthesis in yeast. J. biol. Chem 235:128
    [Google Scholar]
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