1887

Abstract

SUMMARY:

Isolates from the rumens of sheep of presumptively identified , species of Bacteroides, Selenomonas, Butyrivibrio, Bacillus, Eubacterium, Clostridium and Gram-positive cocci were found to be proteolytic. Some of these strains also had exopeptidase and amidase activity, but significant deaminase activity was rare. Most of the strains preferentially utilized ammonia in synthesizing cellular constituents in media containing preformed amino acids. Few of the strains had urease activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-3-461
1963-06-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/3/mic-31-3-461.html?itemId=/content/journal/micro/10.1099/00221287-31-3-461&mimeType=html&fmt=ahah

References

  1. Abou Akkada A. R., Howard B. H. 1960; The biochemistry of rumen protozoa. 3. The carbohydrate metabolism of Entodinium. Biochem. J. 76:445
    [Google Scholar]
  2. Abou Akkada A. R., Howard B. H. 1962; The biochemistry of rumen protozoa. 5. The nitrogen metabolism of Entodinium. Biochem. J. 82:313
    [Google Scholar]
  3. Annison E. F. 1956; Nitrogen metabolism in the sheep. Protein digestion in the rumen. Biochem. J. 64:705
    [Google Scholar]
  4. Blackburn T. H., Hobson P. N. 1960a; Proteolysis in the sheep rumen by whole and fractionated rumen contents. J. gen. Microbiol. 22:272
    [Google Scholar]
  5. Blackburn T. H., Hobson P. N. 1960b; Isolation of proteolytic bacteria from the sheep rumen. J. gen. Microbiol. 22:282
    [Google Scholar]
  6. Blackburn T. H., Hobson P. N. 1962; Further studies on the isolation of proteolytic bacteria from the sheep rumen. J. gen. Microbiol. 29:69
    [Google Scholar]
  7. Bladen H. A., Bryant M. P., Doetsch R. N. 1961; A study of bacterial species from the rumen which produce ammonia from protein hydrolyzate. Appl. Microbiol. 9:175
    [Google Scholar]
  8. Bryant M. P. 1959; Bacterial species of the rumen. Bact. Rev. 23:125
    [Google Scholar]
  9. Burness A. T., King H. K. 1958; Detection of fatty acids on paper chromatograms by means of ninhydrin. Biochem. J. 68:32
    [Google Scholar]
  10. Conway E. J. 1957 Microdiffusion Analysis and Volumetric Error, 4th ed.. London: Crosby Lockwood and Son, Ltd;
  11. Eadie J. M., Hobson P. N. 1962; Effect of the presence or absence of rumen ciliate protozoa on the total rumen bacterial count in lambs. Nature, Lond. 193:503
    [Google Scholar]
  12. Fulghum R. S., King K. W., Moore W. E. C. 1958; Development and comparison of media for the isolation of proteolytic bacteria from the rumen. Bact. Proc. 1958:22
    [Google Scholar]
  13. Grant W. M. 1947; Colorimetric micro-method for determination of formic acid. Analyt. Chem. 19:206
    [Google Scholar]
  14. Hobson P. N., Mann S. O. 1961; The isolation of glycerol-fermenting and lipolytic bacteria from the rumen of sheep. J. gen. Microbiol. 25:227
    [Google Scholar]
  15. Hale W. H. 1956; Rumen metabolism of non-protein nitrogen. J. Agric. Fd Chem. 4:948
    [Google Scholar]
  16. Lampson G. P., Singher H. O. 1960; Colorimetric determination of amino acids in presence of a peptide. Proc. Soc. exp. Biol., N.Y. 103:368
    [Google Scholar]
  17. Layne E. 1957 Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzymology 3 New York: Academic Press, Inc;
    [Google Scholar]
  18. McKenzie H. A., Wallace H. S. 1954; The Kjeldahl determination of nitrogen. A critical study of digestion conditions—temperature, catalyst and oxidizing agent. Aust. J. Chem. 7:55
    [Google Scholar]
  19. Stark J. B., Goodban A. E., Owens H. S. 1951; Paper chromatography of organic acids. Analyt. Chem. 23:413
    [Google Scholar]
  20. Van Slyke D. D., Dillon R. T., MacFadyen D. A., Hamilton P. 1941; Gasometric determination of carboxyl groups in free amino acids. J. biol. chem. 141:627
    [Google Scholar]
  21. Warner A. C. I. 1955 Ph.D. Dissertation, Aberdeen University Some aspects of the nitrogen metabolism of the micro-organisms of the rumen with special reference to proteolysis.
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-3-461
Loading
/content/journal/micro/10.1099/00221287-31-3-461
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error