1887

Abstract

SUMMARY:

The effect of various growth substrates on the synthesis of three inducible enzymes, the stereo-specific tartrate dehydratases, has been followed in strains of Pseudomonas. Four organic acids (succinate, malate, acetate, -tartrate) inhibited induction of some or of all of the tartrate dehydratases, while three (pyruvate, -tartrate, and -tartrate) did not inhibit. Whenever inhibition occurred, synthesis of - and -tartrate dehydratase was repressed much more strongly than synthesis of -tartrate dehydratase. Pre-induction of the bacteria did not prevent subsequent repression and a degree of correlation was found between the growth rate supported by substrates and the extent of repression. The significance of the different degrees of repression given by substrates feeding into closely related sites of the Krebs cycle and of the differences in repression exerted on enzymes acting on closely related compounds is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-3-413
1963-06-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/3/mic-31-3-413.html?itemId=/content/journal/micro/10.1099/00221287-31-3-413&mimeType=html&fmt=ahah

References

  1. Brown D. D., Monod J. 1961; Carbon source repression of 6 galactosidase in Escherichia coli.. Fed. Proc. 20:222
    [Google Scholar]
  2. Cohn M., Horibata K. 1959; Physiology of the inhibition by glucose of the induced synthesis of the β galactosidase enzyme system in Escherichia coli.. J. Bact. 78:624
    [Google Scholar]
  3. Engelsberg E., Watson J. A., Hoffe P. A. 1961; The glucose effect and the relationship between glucose permease, acid phosphatase and glucose resistance. Cold. Spr. Horb. Symp. quant. Biol. 26:261
    [Google Scholar]
  4. Friedman T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine. J. Biol. Chem. 147:415
    [Google Scholar]
  5. Lowry O. H., Rosebrough N. J., Farr A. L., Randall A. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  6. MacQuillan A. M., Halvorson H. O. 1962; Metabolic control of β galactosidase synthesis in yeasts. J. Bact. 84:23
    [Google Scholar]
  7. Magasanik B. 1961; Catabolite repression. Cold. Spr. Harb. Symp. quant. Biol. 26:249
    [Google Scholar]
  8. Mandelstam J. 1961; Induction and repression of β-galactosidase in non-growing Escherichia coli.. Biochem. J. 79:489
    [Google Scholar]
  9. Mandelstam J. 1962; The repression of constitutive β-galactosidase in Escherichia coli by glucose and other carbon sources. Biochem. J. 82:489
    [Google Scholar]
  10. Matchett J. R., Legault R. R., Nimmo C. C., Notter G. K. 1944; Tartrates from grape wastes. Industr. Engng Chem. (Industr.) 36:851
    [Google Scholar]
  11. Monod J. 1942 Recherches sur la croissance des cultures bactérienne. Paris: Hermann et Cie;
  12. Neidhardt F. C., Magasanik B. 1957; Reversal of the glucose inhibition of histidase biosynthesis in Aerobacter aerogenes. J. Bact. 73:253
    [Google Scholar]
  13. Neidhardt F. C. 1960; Mutants of Aerobacter aerogenes lacking glucose repression. J. Bact. 80:536
    [Google Scholar]
  14. Repaske R. 1958; Lysis of gram negative organisms and the role of versene. Biochim. biophys. Acta 30:225
    [Google Scholar]
  15. Rosenberger R. F., Shilo M. 1961; Diauxie in tartrate utilizing strains of Pseudomonas and its control by oxaloacetate. Biochem. biophys. Res. Comm. 4:414
    [Google Scholar]
  16. Shilo M. 1957; The enzymic conversion of the tartaric acids to oxaloacetic acid. J. gen. Microbiol. 16:472
    [Google Scholar]
  17. Shllo M., Stanler R. Y. 1957; The utilization of the tartaric acids by Pseudomonas. J. gen. Microbiol. 16:482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-3-413
Loading
/content/journal/micro/10.1099/00221287-31-3-413
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error