1887

Abstract

SUMMARY:

The effect of chemical sensitizing and protective agents on the rate of mutation induction by gamma radiation in strain WP2 of B/r was studied. This organism will not grow in the absence of tryptophan but it mutates spontaneously at a very low rate, and under the influence of radiation at a much higher rate, to stable forms which can grow in the absence of tryptophan. Both inactivation and mutation-induction were apparently related to radiation dose by an exponential function in the absence of modifying agents; in their presence this was not always the case. The sensitizing agents oxygen and AT-ethylmaleimide, and the protective agents cysteine, glycerol, dimethyl sulphoxide and thiourea, affected both the inactivating and mutation-inducing actions of radiation though not always to the same extent.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-3-405
1963-06-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/3/mic-31-3-405.html?itemId=/content/journal/micro/10.1099/00221287-31-3-405&mimeType=html&fmt=ahah

References

  1. Alper T., Gillies N. E. 1958; Dependence of the observed oxygen effect on the post-irradiation treatment of micro-organisms. Nature, Land. 181:961
    [Google Scholar]
  2. Anderson E. H. 1957; The effect of oxygen on mutation induction by X-rays. Proc. nat. Acad. Sci., Wash. 37:340
    [Google Scholar]
  3. Bridges B. A. 1960; Sensitization of Escherichia coli to gamma radiation by JV-ethyl-maleimide. Nature, Land. 188:415
    [Google Scholar]
  4. Bridges B. A. 1961; The effect of N-ethylmaleimide on the radiation sensitivity of bacteria. J. gen. Microbiol. 26:467
    [Google Scholar]
  5. Bridges B. A. 1962a; The chemical sensitization of Pseudomonas species to ionizing radiation. Radiation Res. 16:232
    [Google Scholar]
  6. Bridges B. A. 1962b; Protection of Pseudomonas sp. against gamma radiation by dimethyl sulphoxide. Int. J. radiation Biol. 5:101
    [Google Scholar]
  7. Bridges B. A. 1962; The chemical protection of Pseudomonas species against ionizing radiation. Radiation Res. 17:801
    [Google Scholar]
  8. Burdette W. J. 1961; Influence of penicillin on frequency of induced mutations. Broc. nat. Acad. Sci., Wash. 47:1813
    [Google Scholar]
  9. Cromroy H. L., Adler H. I. 1962; Influences of β-mercaptoethylamine and oxygen removal on the X-ray sensitivity of four strains of Escherichia coli.. J. gen. Microbiol. 28:431
    [Google Scholar]
  10. Dewey D. L. 1962; X-ray inactivation of inducible enzyme synthesis and the effect of oxygen and glycerol. Nature, Lond. 194:158
    [Google Scholar]
  11. Dewey D. L. 1963; The X-ray sensitivity of Serratia marcescens. Radiation Res. in the Press
  12. Freeman B. M., Bridges B. A. 1960; Suitability of various plating media for counting bacteria after exposure to gamma radiation. Int. J. appl. Radiation and Isotopes 8:186
    [Google Scholar]
  13. Haas F. L., Doudney C. O. 1957; A relation of nucleic acid synthesis to radiation-induced mutation frequency in bacteria. Proc. nat. Acad. Set., Wash. 43:871
    [Google Scholar]
  14. Hill R. F. 1962; Ultraviolet-induced reversions from auxotrophy in Escherichia coli.. J. gen. Microbiol in the Press
  15. Hollaender A., Billen D., Doudney C. O. 1956; The modification of X-ray-induced mutations in Escherichia coli by pre- and post-treatment. Radiation Res. 5:235
    [Google Scholar]
  16. Kada T., Bbuk E., Mabcovich H. 1960; Comparaison de l’induction de mutants prototrophes par les rayons × et u.v. chez Escherichia coli B/r Try-. Ann. Inst. Pasteur 99:547
    [Google Scholar]
  17. Kada T., Doudney C. O., Haas F. L. 1961; Some biochemical factors in X-ray-induced mutation in bacteria. Genetics 46:683
    [Google Scholar]
  18. Stapleton G. E., Sbabra A. J., Hollaender A. 1955; Some nutritional aspects of bacterial recovery from ionizing radiations. J. Bact. 70:7
    [Google Scholar]
/content/journal/micro/10.1099/00221287-31-3-405
Loading
/content/journal/micro/10.1099/00221287-31-3-405
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error