1887

Abstract

SUMMARY: The mechanism by which 11-deoxycorticosterone (DOC) inhibits the growth of was studied by examining the effects of this steroid upon metabolism by the intact mycelium. This compound inhibited the rate of endogenous respiration and the rate of uptake of glucose from the medium, but no evidence was found for the release of intracellular components from the mycelium. Incubation of mycelium in the presence of [C]-glucose resulted in the incorporation of [C] into 37 components within 3 min. in aqueous ethanol extracts of mycelium, although 98% of the [C] in these extracts was contained in fructose, alanine, phosphorylated compounds and an unidentified compound. Incubation in the presence of 11-deoxycorticosterone resulted in a marked change in distribution, with decrease of the labelling of the phosphorylated compounds and fructose and increases in the labelling of the alanine and unidentified component. The results show considerable similarity to the effects of agents such as 2, 4-dinitrophenol on yeast metabolism and suggest that the uncoupling of phosphorylation from oxidation may be one result of the action of 11-deoxycorticosterone on

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-30-2-261
1963-02-01
2019-12-13
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-30-2-261
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error