1887

Abstract

SUMMARY:

The mechanism by which 11-deoxycorticosterone (DOC) inhibits the growth of was studied by examining the effects of this steroid upon metabolism by the intact mycelium. This compound inhibited the rate of endogenous respiration and the rate of uptake of glucose from the medium, but no evidence was found for the release of intracellular components from the mycelium. Incubation of mycelium in the presence of [C]-glucose resulted in the incorporation of [C] into 37 components within 3 min. in aqueous ethanol extracts of mycelium, although 98% of the [C] in these extracts was contained in fructose, alanine, phosphorylated compounds and an unidentified compound. Incubation in the presence of 11-deoxycorticosterone resulted in a marked change in distribution, with decrease of the labelling of the phosphorylated compounds and fructose and increases in the labelling of the alanine and unidentified component. The results show considerable similarity to the effects of agents such as 2, 4-dinitrophenol on yeast metabolism and suggest that the uncoupling of phosphorylation from oxidation may be one result of the action of 11-deoxycorticosterone on

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/content/journal/micro/10.1099/00221287-30-2-261
1963-02-01
2021-08-03
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References

  1. Baar S., Bull J. P. 1953; Salt interference in sugar chromatography of urine. Nature, Lond 172:414
    [Google Scholar]
  2. Bassham J. A., Calvin M. 1957 The Path of Carbon in Photosynthesis New Jersey: Prentice Hall Inc;
    [Google Scholar]
  3. Benson Α. Α., Bassham J. Α., Calvin M., Goodale T. C, Haas V. A., Stepka W. 1950; The path of carbon in photosynthesis. V. Paper chromatography and radio-autography of the products. J. Amer. Chem. Soc 72:1710
    [Google Scholar]
  4. Chattaway F. W., Townsley J. D., Barlow A. J. E. 1959; Effect of steroids and related compounds on the growth of dermatophytes. Nature, Lond 184:1731
    [Google Scholar]
  5. Chattaway F. W., Townsley J. D., Barlow A. J. E. 1962; The effect of certain steroids upon the growth of Trichophyton rubrum. J. gen. Microbiol 28:437
    [Google Scholar]
  6. Conner R. L. 1957; Interaction of stigmasterol and 2,4-dinitrophenol in the growth of Tetrahymena piriformis. Science 126:698
    [Google Scholar]
  7. Conner R. L. 1959; Inhibition of growth of Tetrahymena piriformis by certain steroids. J. gen. Microbiol 21:180
    [Google Scholar]
  8. Fiske C. H., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. Mol. Chem 66:375
    [Google Scholar]
  9. Hardy T. L., Holland D. O., Naylor J. H. C. 1955; One-phase solvent mixtures for the separation of amino acids. Analyt. Chem 27:971
    [Google Scholar]
  10. Heppel L. A. 1955 Methods of Enzymology 2530 Colowick S. P., Kaplan M. O. New York: Academic Press Inc;
    [Google Scholar]
  11. Jermyn M. A., Isherwood F. A. 1949; Improved separation of sugars on the paper partition chromatogram. Biochem. J 44:402
    [Google Scholar]
  12. Kornberg H. L. 1958; The metabolism of Ca compounds in micro-organisms. 1. The incorporation of 2-[14C]acetate by Pseudomonas fluorescens, and by a corynebacterium, grown on ammonium acetate. Biochem. J 68:535
    [Google Scholar]
  13. Lester G., Hechter O. 1959; The relationship of sodium, potassium and deoxycorticosterone in Neurospora crassa. Proc. nat. Acad. Sci., Wash 45:1792
    [Google Scholar]
  14. Lester G., Stone D., Hechter O. 1958; The effect of deoxycorticosterone and other steroids on Neurospora crassa. Arch. Biochem. Biophys 75:196
    [Google Scholar]
  15. Mahler H. R. 1955 Methods in Enzymology 2688 Colowick S. P., Kaplan M. O. New York: Academic Press Inc;
    [Google Scholar]
  16. Moore S., Stein W. H. 1954; A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. biol. Chem 211:907
    [Google Scholar]
  17. Moses V. 1958; [14C]-glucose metabolism in fungal cells. J. gen. Microbiol 20:184
    [Google Scholar]
  18. Moses V., Smith M. J. H. 1960; Uncoupling reagents and metabolism. 2. Effects of 2,4-dinitrophenol and salicylate on glucose metabolism in yeast. Biochem. J 76:585
    [Google Scholar]
  19. Shacter B. 1949; Increase in yeast respiration in presence of several steroids and diethylstilboestrol. Arch. Biochem 23:321
    [Google Scholar]
  20. Smith I. 1960 Chromatographic and Electrophoretic Techniques 1 London: Wm. Heinemann Medical Books Ltd;
    [Google Scholar]
  21. Townsley J. D., Chattaway F. W., Barlow A. J. E. 1961; The effect of 11-deoxy-corticosterone on carbohydrate metabolism in Trichophyton rubrum. Biochem. J 81:14P
    [Google Scholar]
  22. Yielding K. L., Tomkins G. M. 1959; Inhibition of the enzymic oxidation of DPNH by steroid hormones. Proc. nat. Acad. Sci., Wash 45:1730
    [Google Scholar]
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