1887

Abstract

SUMMARY: All strains of and tested, were found to contain a blue protein; this has been named ‘Azurin’. It has been extracted from the cells of all three species and it has also been isolated from culture supernatant fluids of . The azurin was purified by chromatography on ion-exchange cellulose derivatives and obtained in physically pure form; the materials from each species were identical. In one strain of azurin comprised 0·1% of the bacterial dry weight. Azurin is a water-soluble autoxidizable protein of high oxidation-reduction potential (+395 mV.). It has an absorption maximum at 625 mμ in the oxidized form, with a millimolar extinction coefficient of 3·5 × 10. It contains 0·45% copper which can be completely removed by dialysis against cyanide. The molecular weight is 14,600 ( = 1·58 × 10, = 10·6 × 10). Azurin can be reduced by cysteine, glutathione and other reducing agents; the blue colour disappears on reduction. Azurin undergoes reduction in the presence of concentrated cell-free extracts of and succinate, and is reoxidized on aeration.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-30-1-105
1963-01-01
2019-10-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-30-1-105
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error