1887

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Volume 3, Issue 1

The Isolation and Cultivation of Sulphate-Reducing Bacteria Free

Abstract

Summary: There are many strains and perhaps several species of sulphate-reducing bacteria. They may be isolated by using a variety of media over a wide range of temperature. Crude cultures are readily obtained, but isolation of absolutely pure cultures is usually difficult. Considerable simplification in procedure may sometimes be effected by including 3% NaSO.7HO in the media; the sulphite eliminates most of the contaminating organisms in crude cultures and facilitates subsequent purification. A method of maintaining stock cultures on sterile clay is preferred to the use of artificial media.

No growth takes place in media rendered ‘biologically free’ of iron, but the traces normally present in media constituents as impurities are sufficient for good development. Rapid and abundant growth free from ferrous sulphide has been obtained in a mineral salts + lactate + yeast extract medium containing ferrous ions below the saturation concentration for ferrous sulphide.

One thermophilic and four mesophilie strains have been shown to be facultative autotrophs, using the oxidation of hydrogen as energy source, with sulphate, sulphite, thiosulphate and elementary sulphur as hydrogen acceptors. No autotrophic growth takes place in the absence of hydrogen. Hydrogen can be provided by immersing metallic iron in the medium.

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© Society for General Microbiology, 1949
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1949-01-01
2023-12-10
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References

  1. Baars J.K. 1930; Over Sulfaatreductie door Bacterien. Dissertation. W. D. Meinema, N. V., Delft.:
     [Google Scholar]
  2. Beijerinck W.M. 1895; Über Spirillum desulfuricans als Ursache von Sulfat-reduktion. Zbl. BaJct.(2. Abt.) 1:49–104
     [Google Scholar]
  3. Bergey. 1939 Manual of Determinative Bacteriology Bergey D.H., Breed R.S., Murray E.G., Hitchens A.P. ed. , 5th ed.. Baltimore: Williams and Wilkins.;
     [Google Scholar]
  4. Bergey. 1948 Manual of Determinative Bacteriology Breed R.S., Murray E.G.D., Hitchens A.P. ed. , 6th ed.. Baltimore: Williams and Wilkins.;
     [Google Scholar]
  5. Bunker H.J. 1936 A Review of the Physiology and Biochemistry of the Sulphur Bacteria. Dep.Sci.Industr.Res., Chem.Res.Special Rep. 3 London: H.M.Stat.Office;
     [Google Scholar]
  6. Bunker H.J. 1938; Microbiological experiments in anaerobic corrosion. J.Soc.chem.Ind., Lond. 58:93
     [Google Scholar]
  7. Bunker H.J. 1939; Factors influencing the growth of Vibrio desulphuricans. Abstr.Comm.3rd Int.Congr.Microbiol p. 64
     [Google Scholar]
  8. Butlin K.R., Adams M.E. 1947; Autotrophic growth of sulphate-reducing bacteria. Nature; London: 160154
     [Google Scholar]
  9. Copenhagen W.J. 1934 Occurrence of Sulphides in Certain Areas of the Sea Bottom on the South African Coast. Union S.Afr.Dep.Comm, and Ind., Fish and Marine Biol.Survey Div., Investigational Report no.3
    [Google Scholar]
  10. Czurda V. 1940; Zur Kenntnis der bakteriellen Sulfat-reduktion. Arch.Mikrobiol. 11:187
     [Google Scholar]
  11. Datta S.C. 1943; Production of elementary sulphur by reduction of sulphate through bacterial agency. Curr.Sci. 12:305
     [Google Scholar]
  12. Delden Van A. 1903; Beitrag zur Kenntnis der Sulfat-reduktion durch Bakterien. Zbl.Bakt. (2.Abt.) 11:31–113
     [Google Scholar]
  13. Elion L. 1925; A thermophilic sulphate-reducing bacterium. Zbl.Bakt. (2.Abt.) 63:58
     [Google Scholar]
  14. Gahl R., Anderson B. 1928; Sulphate-reducing bacteria in California oil waters. Zbl.Bakt. (2.Abt.) 73:331
     [Google Scholar]
  15. Hadley R.F. 1943 The Influence of Sporovibrio desulphuricans on the Current and Potential Behaviour of Corroding Iron. Philadelphia: Susquehanna Pipe Line Company.;
     [Google Scholar]
  16. Kligler I.J., Guggenheim K. 1938; The influence of vitamin C on the growth of anaerobes in the presence of air. J.Bact. 35:141
     [Google Scholar]
  17. Kluyver A.J. 1931 The Chemical Activities of Micro-organisms. London: University of London Press.;
     [Google Scholar]
  18. Kluyver A.J., Van Niel C.B. 1936; Prospects for a natural system of classification of bacteria. Zbl.Bakt. (2.Abt.) 94:369
     [Google Scholar]
  19. Knaysi G. 1943; Acytological and microchemical study of Thiobacillus thiooxidans. J.Bact. 46:451
     [Google Scholar]
  20. Kühr C.A.H., Von Wolzogen & van der Vlugt I.S. 1934; The graphitization of cast iron as an eleetrobiochemical process in anaerobic soils. Water 18:147
     [Google Scholar]
  21. LePage G.A., Umbreit W.W. 1943; The occurrence of adenosine-3-triphosphate in autotrophic bacteria. J.biol.Chem. 148:255
     [Google Scholar]
  22. Niel Van C.B. 1943; Biochemical problems of the chemoautotrophic bacteria. Physiol.Rev. 23:338
     [Google Scholar]
  23. Pont E.G. 1939; Association of sulphate reduction in the soil with anaerobic iron corrosion. J.Aust.Inst, agric.Sci. 5:170
     [Google Scholar]
  24. Rittenberg S.C. 1941; Studies on marine sulfate-reducing bacteria. Thesis Univ.California.;
     [Google Scholar]
  25. Starkey R.L. 1938; A study of spore formation and other morphological characteristics of Vibrio desulphuricans. Arch.Mikrobiol. 9:268
     [Google Scholar]
  26. Starkey R.L., Wight K.M. 1945 Anaerobic Corrosion of Iron in Soil. Final report of the American Gas Ass.Iron Corrosion Res.Fellowship, Amer.Gas Ass. New York.;
     [Google Scholar]
  27. Stephenson M. 1947; Some aspects of hydrogen transfer. Ant.van Leeuwenhoek. 12:31
     [Google Scholar]
  28. Stephenson M., Stickland L. 1931; The reduction of sulphate to sulphide by molecular hydrogen. Biochem.J. 25:215
     [Google Scholar]
  29. Tanner F.W. 1918; Studies on the bacterial metabolism of sulphur.II.Formation of hydrogen sulphide from certain sulphur compounds by yeast-like fungi. J.Amer.chem.Soc. 40:663
     [Google Scholar]
  30. Tausson W.A., Alioschina W.A. 1932; Über die Bakterielle Sulfatreduktion bei Anwesenheit der Kohlenwasserstoffe. Mikrobiology (U.S.S.R.) 1:229
     [Google Scholar]
  31. Umbreit W.W., Vogel H.R., Vogler K.G. 1942; The significance of fat in sulphur oxidation by Thiobacillus thiooxidans. J.Bact. 43:141
     [Google Scholar]
  32. Waring W.S., Werkman C.H. 1943; Growth of bacteria in an iron-free medium. Arch.Biochem. 1:303
     [Google Scholar]
  33. Zobell C.E. 1945; The role of bacteria in the formation and transformation of petroleum hydrocarbons. Science 102:364
     [Google Scholar]
  34. Zobell C.E. 1946a; Marine Microbiology. Waltham, Mass. Chronica: Botanica Company.;
     [Google Scholar]
  35. Zobell C.E. 1946b; Action of micro-organisms on hydrocarbons. Bact.Rev. 10:1
     [Google Scholar]
  36. Zobell C.E. 1947; Bacterial release of oil from oil-bearing materials. World Oil 126:36127–35
     [Google Scholar]
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