1887

Abstract

SUMMARY: Cultures of the giant bacterium Peshkoff, among them our own isolates from cow dung, were studied. A method for isolation is described. A fluid medium containing acid-hydrolysed casein, thiamine, biotin, sodium acetate, sodium butyrate and dipotassium phosphate, at pH 7.8, was devised which, without added colloids, allowed luxuriant growth of when sufficient aeration was provided. has been found to utilize acetic, butyric and β-hydroxybutyric acids. A variety of other carbon sources did not appear to be readily utilized by The organism produced a carotenoid-like yellow pigment, the synthesis of which was inhibited by diphenylamine. A large amount of poly-β-hydroxybutyric acid (PHB) was synthesized by growing in the fluid medium described above. Much of the PHB was liberated as free granules. was found to be Gram-positive, in contrast to previously published reports.

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1962-07-01
2022-01-22
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References

  1. Bisset K. A. 1953; The cytology of Caryophanon latum. Mycologia 45:790
    [Google Scholar]
  2. DeLamater E. D. 1952; Preliminary observations on the occurrence of mitosis in Caryophanon latum. Mycologia 44:203
    [Google Scholar]
  3. Doudoroff N., Stanier R. Y. 1959; Role of poly-β-hydroxybutyric acid in the assimilation of organic carbon by bacteria. Nature, Lond. 183:1440
    [Google Scholar]
  4. Ellinghausen H. C., Pelczar M. J. 1957; Effect of diphenylamine on pigment production by Neisseria. J. Bact. 73:130
    [Google Scholar]
  5. Forsyth W. G. C., Hayward A. C., Roberts J. B. 1958; Occurrence of poly-β-hydroxybutyric acid in aerobic Gram negative bacteria. Nature, Lond. 182:800
    [Google Scholar]
  6. Herbert D. 1961; The chemical composition of micro-organisms as a function of their environment. Symp. Soc. gen. Microbiol. 11:391
    [Google Scholar]
  7. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. J. Bact. 82:33
    [Google Scholar]
  8. Lemoigne M. 1927; fitudes sur l’autolyse microbienne. Origine de l’acide β -oxybutyrique forme par autolyse. Ann. Inst. Pasteur 41:148
    [Google Scholar]
  9. Levine H. B., Wolochow H. 1959; Occurrence of poly-β-hydroxybutyrate in Pseudomonas pseudomallei. J. Bact. 79:305
    [Google Scholar]
  10. Manual of Microbiological Methods 1957; Society of American Bacteriologists. New York, N.Y.: McGraw-Hill;
    [Google Scholar]
  11. May P. S., Gershenfeld L. 1955; Caryophanon latum—the effect of selected nutrients on its growth in a liquid synthetic medium. Bact. Proc.48
    [Google Scholar]
  12. Murray R. G. E. 1957; Direct evidence for a cytoplasmic membrane in sectioned bacteria. Canad. J. Microbiol. 3:531
    [Google Scholar]
  13. Peshkoff M. A. 1940; Phylogenesis of new microbes, Caryophanon latum and Caryophanon tenue, organisms which are intermediate between the blue-green algae and the bacteria. (Summary in English.). J. gen. Biol., Moscow 1:598
    [Google Scholar]
  14. Peshkoff M. A. 1946; Fine structure and mechanism of division of the ‘nuclei’ of the bacterium Caryophanon latum. Nature, Lond. 157:137
    [Google Scholar]
  15. Pringsheim E. G., Robinow C. F. 1947; Observations on two very large bacteria, Caryophanon latum Peshkoff and IAneola longa (nomen provisorium). J. gen. Microbiol. 1:267
    [Google Scholar]
  16. Sall T., Mudd S. 1955; A cytological study of Caryophanon latum. J. gen. Microbiol. 12:47
    [Google Scholar]
  17. Slepecky R. A., Law J. H. 1960; A rapid spectrophotometric assay of α-, β -un-saturated acids and β -hydroxy acids. Analyt. Chem. 32:1697
    [Google Scholar]
  18. Tomcsik J., Grace J. B. 1955; Bacterial cell walls as revealed by the specific cell-wall reaction and by direct staining with alcian blue. J. gen. Microbiol. 13:105
    [Google Scholar]
  19. Tuffery A. A. 1953; The morphology and systematic position of Caryophanon. Proc. 6th int. Congr. Microbiol. 1:77
    [Google Scholar]
  20. Weeks O. B., Kelley L. M. 1958; Observations on the growth of the bacterium Caryophanon latum. J. Bact. 75:326
    [Google Scholar]
  21. Williamson D. H., Wilkinson J. F. 1958; The isolation and estimation of the poly-β-hydroxybutyrate inclusions of Bacillus species. J. gen. Microbiol. 19:198
    [Google Scholar]
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