1887

Abstract

SUMMARY: Rod-shaped particles suspended in a liquid undergo a partial orientation during flow; the flowing suspension has the property of scattering light mainly in certain preferred directions. The elementary theory of the phenomenon shows that it can be applied practically as a photometric method of following changes in length. A simple and effective apparatus for this purpose is described, and its performance illustrated. A brief discussion of size changes in synchronized cultures indicates the relation between these changes and the rate of fission.

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/content/journal/micro/10.1099/00221287-27-3-489
1962-03-01
2024-10-10
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References

  1. Abbo F., Pardee A. B. 1960; Synthesis of macromolecules in synchronously dividing bacteria. Biochim. biophys. Acta 39:478
    [Google Scholar]
  2. van de Hulst H. C. 1957 Light Scattering by Small Particles New York: John Wiley and Sons, Inc;
    [Google Scholar]
  3. Norris K. P., Powell E. O. 1961; Improvements in determining total counts of bacteria. J. R. micr. Soc 80:107
    [Google Scholar]
  4. Powell E. O. 1958; An outline of the pattern of bacterial generation times. J. gen. Microbiol 18:382
    [Google Scholar]
  5. Powell E. O. 1962; Photometric methods in bacteriology. J. Sci. Fd Agric (In the Press.)
    [Google Scholar]
  6. Sadron C. 1953; Methods of determining the form and dimensions of particles in solution: A critical survey. In Progress in Biophysics 3237 Ed. Butler J. A. V., Randall J. T. London: The Pergamon Press, Ltd;
    [Google Scholar]
  7. Siedentopf H. 1912; t)ber ultramikroskopische Abbildung linearer Objekte. Z. wiss. Mikr 29:1
    [Google Scholar]
  8. Sowerby J. McG. 1944; A photoelectric photometer for measuring the light scattered by the surface of a transparent material. J. sci. Instrum 21:42
    [Google Scholar]
  9. Stadie F. 1928; Probleme der Brownschen Molekularbewegung. Ann. Phys. Lpz 86:751
    [Google Scholar]
  10. Stárka J., Koza J. 1959; Nephelometric determination of cell count in synchronously dividing cultures of bacteria. Biochim. biophys. Acta 32:261
    [Google Scholar]
  11. Wippler C. 1954; Étude théorique de la diffusion de la lumière par les sols de batonnets orientés. J. Chim. phys 51:122
    [Google Scholar]
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