1887

Abstract

SUMMARY: Phospholipase A (100 μfg./ml.) acting at 37° for 30 min. decreased the infective titre of crude or purified Murray Valley encephalitis (MVE) virus from 7 or 8 log to less than 1 log. This inactivated virus yielded 2 log of infective ‘ribonucleic acid’ (‘RNA’) when it was treated with phenol. Ether, chloroform or butanol acting at 0–4° each decreased the infectivity of crude or purified MVE virus. Again the inactivated virus yielded ‘RNA’ with an infective titre higher than the titre of the inactivated virus. Infective ‘RNA’ prepared from crude MVE virus was not inactivated by ether, chloroform or butanol. High concentrations of phospholipase A preparations destroyed ‘RNA’, possibly due to traces of contaminating ribo-nuclease. The results suggest that intact viral phospholipid is necessary for infectivity of the virus particle.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-25-3-451
1961-07-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-25-3-451
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error