SUMMARY: By suitable adjustment of growth conditions in continuous cultures, organisms of B/r with average lengths covering a wide range were produced and studied. From X-ray survival data it was concluded that radiosensitive sites were distributed along the length of an organism at intervals of 1-1.5 μ, whilst nuclear staining by the HCl-Giemsa method showed chromatinic bodies at an average spacing of 1.2 μ. Thus each nuclear body appeared to be a radiosensitive site. Lysozyme treatment did not reveal evidence of transverse membranes apart from those at visible ‘waists’. It was concluded that the longer organisms arose by the inhibition of the terminal stages of division. This inhibition did not affect the multiplication of the nuclear and cytoplasmic components, although there was considerable aggregation of nuclear material in irradiated organisms.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error