1887

Abstract

SUMMARY: The walls and cell contents of a number of lactobacilli were prepared by mechanical disruption of the organisms followed by differential centrifugation. The nature of the teichoic acid present in the separated fractions was determined by extracting it with dilute trichloroacetic acid, precipitating with ethanol and identifying the precipitated polymer by hydrolysis to ribitol or glycerol phosphates and other recognizable degradation products. All the organisms contained a glycerol teichoic acid within the cell. This and similar observations with other bacteria indicates that glycerol teichoic acids play an important part in cellular metabolism. Both glycerol and ribitol teichoic acids occur in walls. The presence and type of teichoic acid in the walls correlate with the serological behaviour of lactobacilli and provide a useful means of classification. It is suggested that teichoic acids themselves may possess antigenic properties.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-24-2-295
1961-02-01
2022-07-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/24/2/mic-24-2-295.html?itemId=/content/journal/micro/10.1099/00221287-24-2-295&mimeType=html&fmt=ahah

References

  1. Armstrong J. J., Baddiley J., Buchanan J. G. 1960; Structure of the ribitolteichoic acid from the walls of Bacillus subtilis . Biochem. J 76:610
    [Google Scholar]
  2. Armstrong J. J., Baddiley J., Buchanan J. G., Carss B., Greenberg G. R. 1958; Isolation and structure of ribitol phosphate derivatives (teichoic acids) from bacterial cell walls. J. chem. Soc p. 4344
    [Google Scholar]
  3. Armstrong J. J., Baddiley J., Buchanan J. G., Davison A. L., Kelemen M. V., Neuhaus F. C. 1959; Composition of teichoic acids from a number of bacterial walls. Nature; Lond: 184247
    [Google Scholar]
  4. Barton-Wright E. C. 1946 Practical Methods for the Microbiological Assay of Vitamin B Complex and Essential Amino Acids London: Ashe Laboratories;
    [Google Scholar]
  5. Ikawa M., Snell E. E. 1960; Cell wall composition of lactic acid bacteria. J. biol. Chem 235:1876
    [Google Scholar]
  6. McCarty M. 1959; The occurrence of polyglycerophosphate as an antigenic component of various Gram-positive bacterial species. J. exp. Med 109:861
    [Google Scholar]
  7. Salton M. R. J., Horne R. W. 1951; Methods of preparation and some properties of cell walls. Biochim. Biophys. Acta 7:177
    [Google Scholar]
  8. Sharpe M. E. 1955; A serological classification of Lactobacilli. J. gen. Microbiol 12:107
    [Google Scholar]
  9. Sharpe M. E. 1957; A note on the source and identification of the strains of Lactobacilli submitted to chromatographic analysis. J. appl. Bact 20:215
    [Google Scholar]
  10. Sharpe M. E. 1959; A note on the source and identification of the strains of thermophilic Lactobacilli submitted to chromatographic analysis. J. appl. Bact 22:325
    [Google Scholar]
  11. Sharpe M. E., Wheater D. M. 1957 Lactobacillus helveticus. J. gen. Microbiol 16:676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-24-2-295
Loading
/content/journal/micro/10.1099/00221287-24-2-295
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error